高效液相色谱法测定化妆品中苯氧乙醇、4-羟基苯甲酸甲酯和4-羟基苯甲酸丙酯  

Determination of phenoxyethanol,methyl 4-hydroxybenzoate,and propyl 4-hydroxybenzoate in cosmetics by high-performance liquid chromatography

作　　者：付慧[1] 张淼[1] FU Hui;ZHANG Miao(National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China)

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境卫生学杂志》2024年第5期448-452,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

摘　　要：目的建立化妆品中苯氧乙醇、4-羟基苯甲酸甲酯和4-羟基苯甲酸丙酯的高效液相色谱测定法。方法样品经甲醇超声提取,0.45μm聚四氟乙烯滤膜过滤,使用ZORBAX SB-C_(18)(150 mm×4.6 mm,5μm)为分析柱,甲醇、乙腈和0.02 mol/L乙酸铵(pH=4.0)为流动相梯度洗脱,通过二极管阵列检测器进行检测。结果苯氧乙醇、4-羟基苯甲酸甲酯和4-羟基苯甲酸丙酯分别在242~20000 mg/kg、14.6~4000 mg/kg和6.6~4000 mg/kg范围内线性关系良好,相关系数均大于0.999,回收率为90.4%~104%,日内、日间精密度小于9.2%,方法应用于化妆品中防腐剂含量测定能力验证实验并获得满意结果。结论该方法简便、快速、准确、稳定,适用于同时定量测定化妆品中的苯氧乙醇、4-羟基苯甲酸甲酯和4-羟基苯甲酸丙酯含量。Objective To establish a method for determining phenoxyethanol,methyl 4-hydroxybenzoate,and propyl 4-hydroxybenzoate in cosmetic products based on high-performance liquid chromatography(HPLC).Methods The samples were extracted by ultrasonic extraction with methanol and filtered by 0.45μm PTFE filter membrane.High-performance liquid chromatography was performed on a ZORBAX SB-C_(18)(150 mm×4.6 mm,5μm)column with a mobile phase of methanol,acetonitrile,and 0.02 mol/L ammonium acetate(pH=4.0)for gradient elution,and a diode array detector was used for determination.Results Phenoxyethanol,methyl 4-hydroxybenzoate,and propyl 4-hydroxybenzoate showed a good linear relationship within the range of 242-20000,14.6-4000,and 6.6-4000 mg/kg,respectively,with a correlation coefficient of>0.999,a recovery rate of 90.4%-104%,and intra-and inter-day precisions of<9.2%.The method was used in the proficiency test to determine the content of preservatives in cosmetics,and satisfactory results were obtained.Conclusion This method is simple,rapid,accurate,and stable and is suitable for the simultaneous determination of phenoxyethanol,methyl 4-hydroxybenzoate,and propyl 4-hydroxybenzoate in cosmetics.

关 键 词：高效液相色谱法 化妆品 防腐剂 苯氧乙醇 4-羟基苯甲酸甲酯 4-羟基苯甲酸丙酯 

分 类 号：O657[理学—分析化学] R113[理学—化学]