胰腺癌免疫基因组学分型、特征及其临床意义研究  

Study on Immunogenomic Typing and Characteristics and Clinical Significance of Pancreatic Cancer

作　　者：曹非 张国耀[1] 史磊[1] 马天江[1] 张磊[1] CAO Fei;ZHANG Guoyao;SHI Lei;MA Tianjiang;ZHANG Lei(Department of Oncology,Luohe Central Hospita,Luohe,Henan Province 462005,Chian)

机构地区：[1]漯河市中心医院肿瘤科,河南漯河462005

出　　处：《吉林医药学院学报》2024年第3期194-199,共6页Journal of Jilin Medical University

基　　金：河南省医学科技公关计划项目(LHGJ20221029)。

摘　　要：目的 通过基因组学建立胰腺癌免疫分型,并进一步探讨其免疫特征和临床意义。方法 癌症基因组图谱数据库下载178例胰腺癌患者的基因表达谱及临床特征数据,通过单样本基因集富集分析(ssGSEA)评分对患者进行分组;采用ESTIMATE及CIBERSORT分别评估各胰腺癌免疫分型样本中免疫微环境及免疫细胞的组成;采用Kaplan-Meier绘制生存曲线并对组间差异进行log-rank检验;GSEA鉴定不同免疫分型GO富集通路。结果 根据基质评分、免疫评分和肿瘤纯度将患者分为高、中、低免疫组,三组间预后具有统计学差异。高免疫组所包含的免疫细胞和基质细胞数量最多,肿瘤纯度最低;HLA、PD-L1(CD274)和CTLA4表达明显增高;浆细胞、活化的CD4^(+)记忆性T细胞、CD8^(+)T细胞以及γδT细胞含量最高,而M0巨噬细胞和M2巨噬细胞最少;总生存率明显低于低免疫组患者;抗免疫活性明显升高。结论 胰腺癌免疫分型有助于判断患者的预后,高免疫组患者具有较高的抗免疫活性和较低的生存率。Objective To establish the immune classification of pancreatic cancer through genomics and further explore its immune characteristics and clinical significance.Methods The gene expression profile and clinical characteristics data of 178 patients with pancreatic cancer were downloaded from the Cancer Genome Map Database,and the patients were grouped by single sample gene set enrichment analysis(ssGSEA)score;The immune microenvironment and the composition of immune cells in pancreatic cancer immunotyping samples were evaluated by ESTIMATE and CIBERSORT respectively;Using Kaplan-Meier to plot survival curves and conducting logrank tests for inter-group differences;GSEA identifies GO enrichment pathways for different immune subtypes.Result Patients were divided into high,medium,and low immune groups based on matrix score,immune score,and tumor purity,with statistical differences in prognosis among the three groups.The high immune group contains the highest number of immune cells and stromal cells,while the tumor purity is the lowest;The expression of HLA,PD-L1(CD274),and CTLA4 was significantly increased;Plasma cells,activated CD4~+ memory T cells,CD8~+ T cells,and γδ The content of T cells is the highest,while M0 macrophages and M2 macrophages are the least;The overall survival rate was significantly lower than that of patients in the low immune group;The anti immune activity was significantly increased.Conclusion Immunophenotyping of pancreatic cancer is helpful to judge the prognosis of patients.Patients in the high immune group have higher anti-immune activity and lower survival rate.

关 键 词：胰腺癌 基因组学 免疫分型 免疫活性 生存率 

分 类 号：R735.9[医药卫生—肿瘤]