HS-GC-MS结合ROAV分析不同品种橘红花挥发性成分  

Detection of Volatile Components in Flos Citri Grandis from Different Plant Varieties by HS-GC-MS Combined with ROAV

作　　者：杨洁慧 杜佳林 李静文 倪晨 刘伟琼 陈泳琪 詹若挺[1,2,3] 严萍 YANG Jie-hui;DU Jia-lin;LI Jing-wen;NI Chen;LIU Wei-qiong;CHEN Yong-qi;ZHAN Ruo-ting;YAN Ping(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;The Research Centre of Chinese Herbal Resource Science and Engineering/Key Laboratory of Chinese Medical Resources from Lingnan(Guangzhou University of Chinese Medicine)/South China Pharmaceutical Research and Development Laboratory,National Engineering and Technology Research Center of Chinese Patent Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525099,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室(广州中医药大学)/国家中成药工程技术研究中心南药研发实验室,广东广州510006 [3]岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名525099

出　　处：《中国现代中药》2024年第4期616-624,共9页Modern Chinese Medicine

基　　金：广东省乡村振兴战略专项(2023KJ148);广州中医药大学2023年校级大学生创新创业训练计划项目(202310572195)。

摘　　要：目的:系统研究不同品种橘红花挥发性成分差异和香气特征。方法:利用顶空-气相色谱-质谱法(HSGC-MS)对4个品种(“光青”“假西洋”“密叶正毛”和“黄龙正毛”)共40批橘红花的挥发性成分进行检测,结合相对气味活度值(ROAV)分析其中的关键香气成分。采用多元统计分析筛选不同品种橘红花显著性差异成分和特征香气成分。结果:HS-GC-MS检测结果显示,从4个品种橘红花中共鉴定出40个挥发性成分,主要包括烯烃类、醇类、酯类、酮类和醛类,其中以柠檬烯、β-月桂烯、芳樟醇、反式-橙花叔醇等为共有且含量较高的成分。通过ROAV确定了4个品种橘红花共有关键香气成分8个。挥发性成分差异分析结果显示,21个差异标志物可用于品种判别,其中β-蒎烯是“假西洋”的特征香气成分,芳樟醇是“密叶正毛”的特征香气成分,苯乙醛、反式-橙花叔醇和邻氨基苯甲酸甲酯是“黄龙正毛”的特征香气成分。结论:HS-GC-MS结合ROAV系统分析可区分不同品种橘红花并鉴定差异性的特征香气成分,该方法可为橘红花的品种判别和资源综合开发利用提供参考。Objective:To systematically compare the volatile components and aroma characteristics of Flos Citri Grandis produced from different plant varieties.Methods:Headspace-gas chromatography-mass spectrometry(HS-GC-MS)was employed to detect the volatile components of 40 batches of Flos Citri Grandis samples produced from four plant varieties("Guangqing""Jiaxiyang""Miye Zhengmao"and"Huanglong Zhengmao"),and the key aroma components were analyzed based on the relative odor activity value(ROAV).The significantly differential components and characteristic aroma components of Flos Citri Grandis from different varieties were screened by multivariate statistical analysis.Results:HS-GC-MS identified 40 volatile components of Flos Citri Grandis from four plant varieties.The volatile components were mainly olefins,alcohols,esters,ketones,and aldehydes,among which limonene,β-myrcene,linalool,and trans-nerolidol were the common components with high content.Eight key aroma components shared by the four varieties were screened out based on ROAV.The difference analysis of volatile components showed that 21 differential markers could be used for variety distinguishing,among whichβ-pinene and linalool were the characteristic aroma components of"Jiaxiyang"and"Miye Zhengmao",respectively,and benzeneacetaldehyde,trans-nerolidol,and methyl anthranilate were the characteristic aroma components of"Huanglong Zhengmao".Conclusion:We combined HS-GC-MS with ROAV to distinguish different varieties of Flos Citri Grandis and identified the differential characteristic aroma components.This study provides reference for variety distinguishing and comprehensive development and utilization of Flos Citri Grandis resources.

关 键 词：橘红花 顶空-气相色谱-质谱法 相对气味活度值 挥发性成分 品种 

分 类 号：R284[医药卫生—中药学]