circ_0086414通过miR498-/PCK1轴对口腔鳞状细胞癌生物学行为的影响  

Effect of circ_0086414 on biological behavior of oral squamous cell carcinoma via miR-498/PCK1 axis

作　　者：李立恒[1] 王蕊[1] 王晓明[1] 张智轶 张璇 张凡[2] LI Li-heng;WANG Rui;WANG Xiao-ming;ZHANG Zhi-yi;ZHNG Xuan;ZHANG Fan(Department of Stomatology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China;Department of Pathology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院口腔科,河北张家口075000 [2]河北北方学院附属第一医院病理科,河北张家口075000

出　　处：《河北医科大学学报》2024年第4期424-433,共10页Journal of Hebei Medical University

基　　金：河北省医学科学研究课题计划(20210802)。

摘　　要：目的研究circ_0086414通过miR-498/PCK1轴对口腔鳞状细胞癌(oral squamous cell carcinomas,OSCC)生物学行为的影响。方法收集手术切除的OSCC组织及配对的癌旁组织27对,实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)检测组织中circ_0086414和PCK1的表达。将SJG-1细胞分为pcDNA组、pcDNA circ_0086414组、miR-498 NC组、miR-498 mimic组、OE-NC组、OE-PCK1组、miR-498 NC+pcDNA组、miR-498 NC+pcDNA circ_0086414组、miR-498 mimic+pcDNA组、miR-498 mimic+pcDNA circ_0086414组,细胞计数试剂盒(cell counting kit,CCK8)法检测SJG-1细胞增殖,流式细胞术检测SJG-1细胞凋亡,Transwell实验检测SJG-1细胞侵袭;蛋白免疫印迹(Western blot,WB)实验检测上皮间质转化情况,双荧光素酶报告基因检测circ_0086414和miR-498及miR-498和PCK1的关系。结果与癌旁组织比较,OSCC组织中circ_0086414相对表达明显降低(P<0.001);与人正常口腔角质细胞HOK比较,人OSCC细胞系HEK293T、SJG-1、SCC-15、HSC-2中circ_0086414相对表达显著降低(P<0.001);与pcDNA组比较,pcDNAcirc_0086414组SJG-1细胞增殖能力明显降低,凋亡率明显升高,侵袭能力明显减少,N-cadherin表达水平显著减少,E-cadherin表达水平明显升高(均P<0.001);与miR-498 NC组比较,miR-498mimic组SJG-1细胞增殖能力明显升高,凋亡率显著降低,侵袭能力明显增加,N-cadherin表达水平显著增加,E-cadherin表达水平明显减少(均P<0.001);与miR-498 NC+pcDNA组比较,miR-498 NC+pcDNAcirc_0086414组SJG-1细胞增殖能力明显降低,凋亡率明显升高,侵袭能力明显降低,N-cadherin表达水平明显降低,E-cadherin表达水平明显升高,miR-498 mimic+pcDNA组SJG-1细胞增殖能力明显升高,凋亡率明显降低,细胞侵袭能力明显升高,N-cadherin表达水平明显升高,E-cadherin表达水平明显降低(均P<0.001),与miR-498 mimic+pcDNA组比较,miR-498 mimic+pcDNAcirc_0086414组的SJG-1细胞增殖能力明显降低,凋亡率明显升高,侵袭能力明显降低,N-cadherin表达水�Objective To study the effect of circ_0086414 on biological behavior of oral squamous cell carcinoma(OSCC)via miR-498/PCK1 axis.Methods In total,27 pairs of surgically resected OSCC tissues and paired paracancerous tissues were collected.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of circ_0086414 and PCK1 in tissues.SJG-1 cells were divided into pcDNA group,pcDNA circ_0086414 group,miR-498 NC group,miR-498 mimic group,OE-NC group,OE-PCK1 group,miR-498 NC+pcDNA group,miR-498 NC+pcDNA circ_0086414 group,miR-498 mimic+pcDNA group,and miR-498 mimic+pcDNA circ_0086414 group.Cell counting kit(CCK8)was used to detect SJG-1 cell proliferation.Flow cytometry was used to detect SJG-1 cell apoptosis,and Transwell assay was used to detect SJG-1 cell invasion.Western bloting(WB)assay was used to detect epithelial messtimal transformation.The relationship between circ_0086414 and miR-498 and between miR-498 and PCK1 was detected by dual luciferase reporter gene.Results The relative expression of circ_0086414 in OSCC tissues was significantly lower than that in paracancerous tissues(P<0.001).Compared with HOK in normal human oral keratinocytes,the expression of circ_0086414 in human OSCC cell lines HEK293T,SJG-1,SCC-15 and HSC-2 was significantly decreased(P<0.001).Compared with pcDNA group,SJG-1 cells in pcDNAcirc_0086414 group had significantly decreased proliferation ability,significantly increased apoptosis rate,significantly decreased invasion ability,and there was significantly decreased N-cadherin expression level,and significantly increased E-cadherin expression level(P<0.001).Compared with miR-498 NC group,miR-498mimic group had significantly increased proliferation ability,significantly decreased apoptosis rate and significantly increased invasion ability in SJG-1 cells,as well as significantly increased expression level of N-cadherin,and significantly decreased expression level of E-cadherin(all P<0.001).Compared with the miR-498 NC+pcDNA group,the proliferation ability of SJG-1 cells in the

关 键 词：口腔肿瘤 癌 鳞状细胞 RNA 环状 

分 类 号：R739.8[医药卫生—肿瘤]