F框/WD-40域蛋白11对三阴性乳腺癌细胞增殖、迁移的影响  

Effect of F-box/WD-40 domain protein 11 on the proliferation and migration of triple-negative breast cancer cells

作　　者：赵娴 刘相萍[2] 宫明凯 王硕[1] 隋爱华[2] 韩亚斐 王海波[1] Zhao Xian;Liu Xiangping;Gong Mingkai;Wang Shuo;Sui Aihua;Han Yafei;Wang Haibo(Breast Disease Center,the Affiliated Hospital of Qingdao University,Qingdao 266003,China;Medical Research Center,the Affiliated Hospital of Qingdao University,Qingdao 266003,China;Department of Breast and Thyroid Surgery,Weifang Traditional Chinese Hospital,Weifang 261000,China)

机构地区：[1]青岛大学附属医院乳腺病诊疗中心,青岛266003 [2]青岛大学附属医院医学研究中心,青岛266003 [3]潍坊市中医医院乳腺外科,潍坊261000

出　　处：《中华内分泌外科杂志（中英文）》2024年第2期184-189,共6页Chinese Journal of Endocrine Surgery

基　　金：山东省自然科学基金(ZR2021MH158、ZR2021MH119);国家自然科学基金(81572616、81772845)。

摘　　要：视频导读乳腺癌是女性最常见的恶性肿瘤,迄今为止,探索三阴性乳腺癌(TNBC)的靶向治疗始终是医学研究的热点。TNBC是一种特殊分子亚型的乳腺癌类型,因其侵袭性强、转移复发风险高且缺乏有效治疗靶点,导致患者预后差,严重威胁女性健康,是临床亟待解决的治疗难题。本文研究FBXW11对TNBC生物学功能活性的影响,将进一步探讨FBXW11影响TNBC功能潜在作用机制,以便为TNBC的靶向治疗提供新的理论依据。Objective To investigate the expression of F-box/WD-40 domain protein 11(FBXW11)in triple-negative breast cancer cell lines MDA-MB-231 and HCC-1937 and its effects on cell proliferation and migration by recombinant plasmid and the small interfering sequence(siRNA).Methods The recombinant plasmid GV141-FBXW11 was constructed.GV141-FBXW11 and the siRNA targeting FBXW11(siRNA-FBXW11)were transfected into MDA-MB-231 and HCC-1937 cells respectively using Lipofectamine 2000.Western blot was used to verify the expression of FBXW11.The effects of FBXW11 protein expression on the proliferation and migration ability of MDA-MB-231 and HCC-1937 cells were investigated by scratching,colony formation and Transwell chamber assays.Independent sample t test was used to compare the means between the two groups,with P<0.05 as statistically significant.Results The restriction endonuclease digestion and DNA sequencing showed that the FBXW11 gene was inserted into GV141 vector successfully.After transfection with GV141-FBXW11 and siRNA-FBXW11 respectively using Lipofectamine 2000,Western blot results showed that FBXW11 protein was significantly higher in the FBXW11 overexpress groups and lower in the siRNA-FBXW11 groups.And the clone formation rate of the control and experimental plates of the two cell lines was respectively:[231,(0.43±0.03)%,(0.26±0.05)%;1937,(0.28±0.05)%,(0.14±0.03)%],the number of cells in the chamber experiments was respectively:[231,(497±24.27),(240.33±20.03);1937,(475.67±23.46),(362.67±21.55)].The cell proliferation and migration capacity were significantly reduced(P<0.05)in the FBXW11 overexpress groups.The siRNA targeting FBXW11 significantly down-regulated the expression level of FBXW11 in MDA-MB-231 and HCC-1937 cells,the proliferation rates of the control group and the experimental group were respectively:[231,(0.05±0.02)%,(0.2±0.06)%;1937,(0.09±0.04)%,(0.2±0.04)%],the number of cells penetrating the membrane was respectively:[231,(590±27.79),(768±23.15);1937,(554±43.48),(737.33±46.83)].That

关 键 词：乳腺癌 三阴性乳腺癌 F框/WD-40域蛋白11 增殖 迁移 

分 类 号：R737.9[医药卫生—肿瘤]