电针联合低频经颅超声刺激对创伤性脑损伤大鼠脑电信号的影响  

Effect of electroacupuncture combined with low-frequency transcranial ultrasound stimulation on the electroencephalographic signals of rats with traumatic brain injury

作　　者：高思淼 韩雪 吴晓光[1] 郑金钰 高芳雯 李葵花[1,2,4] 彭勇[5,6] 刘兰祥[7] Gao Simiao;Han Xue;Wu Xiaoguang;Zheng Jinyu;Gao Fangwen;Li Kuihua;Peng Yong;Liu Lanxiang(Key Laboratory of Nerve Injury and Repair of Hebei Province,Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Biomedical Engineering,Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Neurology,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China;International Research Center for Medical-Industrial Integration of Hebei,Chengde Medical University,Chengde 067000,Hebei Province,China;Institute of Electrical Engineering,Yanshan University,Qinhuangdao 066004,Hebei Province,China;Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province,Yanshan University,Qinhuangdao 066004,Hebei Province,China;First Hospital of Qinhuangdao,Qinhuangdao 066004,Hebei Province,China)

机构地区：[1]承德医学院,河北省神经损伤与修复重点实验室,河北省承德市067000 [2]承德医学院生物医学工程系,河北省承德市067000 [3]承德医学院附属医院神经内科,河北省承德市067000 [4]承德医学院,河北省医工结合国际研究中心,河北省承德市067000 [5]燕山大学电气工程学院,河北省秦皇岛市066004 [6]燕山大学,河北省智能康复及神经调控重点实验室,河北省秦皇岛市066004 [7]秦皇岛市第一医院,河北省秦皇岛市066004

出　　处：《中国组织工程研究》2025年第2期402-408,共7页Chinese Journal of Tissue Engineering Research

基　　金：河北省神经损伤与修复重点实验室开放课题(NJKF202305),项目负责人:李葵花;河北省卫健委医学科学研究课题(20231558),项目负责人:吴晓光;国家自然科学基金(81871029),项目负责人:刘兰祥。

摘　　要：背景:创伤性脑损伤是由头部受到撞击、打击而导致大脑正常功能被破坏的疾病,目前需要寻找有效治疗方式和客观指标,帮助医生判别损伤状况及恢复患者脑功能。目的:探究电针联合低频经颅超声刺激对创伤性脑损伤大鼠脑电信号的影响。方法:将40只6周龄SPF级雄性SD大鼠随机分为假手术组、模型组、电针组、低频经颅超声刺激组和联合组(n=8),后4组采用Feeney自由落体法造模,假手术组只开骨窗而不打击。各干预组均于造模后1 d开始实施干预,电针组进行电针干预,低频经颅超声刺激组进行低频经颅超声刺激干预,联合组进行两者联合干预,共干预7 d。造模后8 h,用改良神经功能缺损评分评定大鼠神经功能缺损情况;干预7 d后观察大鼠Y迷宫自发轮流行为百分比,而后采集脑电信号,利用快速傅里叶变换分解出α、β、θ和δ波段,计算各频段振荡幅值、能量占比百分比以及Lempel-Ziv复杂度、样本熵。结果与结论:①造模后8 h,模型组、电针组、低频经颅超声刺激组和联合组的改良神经功能缺损评分显著高于假手术组(P<0.05);②造模后第7天,模型组的α波、δ波频带振荡幅值、δ波能量占比百分比显著高于假手术组(P<0.05),自发轮流行为百分比、α波、β波能量占比百分比、Lempel-Ziv复杂度、样本熵显著低于假手术组(P<0.05);③与模型组比较,联合组的α波、δ波频带振荡幅值显著下降(P<0.05),电针组、低频经颅超声刺激组、联合组的α波、β波频带能量占比百分比显著升高(P<0.05),δ波能量占比百分比显著下降(P<0.05);④与电针组和低频经颅超声刺激组相比,联合组的δ波能量占比百分比显著降低(P<0.05),自发轮流行为百分比、α波、β波能量占比百分比、Lempel-Zi复杂度、样本熵显著升高(P<0.05);⑤结果显示,创伤性脑损伤大鼠出现脑电信号异常,而电针联合低频经颅超声刺激干预后�BACKGROUND:Traumatic brain injury is a condition in which the normal function of the brain is disrupted by a bump or impact to the head.It is necessary to find effective treatments and objective targets that can help doctors diagnose the injury status and restore the brain function of patients.OBJECTIVE:To explore the effect of electroacupuncture combined with low-frequency transcranial ultrasound stimulation on the electroencephalographic signals of rats with traumatic brain injury.METHODS:Forty 6-week-old SPF male Sprague-Dawley rats were randomly divided into five groups:sham group,model group,electroacupuncture group,lowfrequency transcranial ultrasound stimulation group and combined group(electroacupuncture+low-frequency transcranial ultrasound stimulation),with eight rats in each group.Feeney weight-drop method was used to establish the animal model of traumatic brain injury.In the sham group,the bone window was only opened without impact.Interventions were started at 1 day after modeling.Electroacupuncture in the electroacupuncture group,low-frequency transcranial ultrasound stimulation in the low-frequency transcranial ultrasound stimulation group,and electroacupuncture+low-frequency transcranial ultrasound stimulation in the combined group were performed for days in total.The modified neurological severity scale score for assessing rats’neurological deficits was performed at 8 hours after modeling.The percentage of spontaneous alternation behavior in the Y-maze was measured at 7 days after modeling.Then,the electroencephalographic signals were collected and electroencephalographic data ofα,β,θ,andδwaves were extracted by fast Fourier transform,and the value of oscillation amplitude and energy ratio were calculated inα,β,θ,andδwaves,as well as the Lempel-Ziv complexity and sample entropy.RESULTS AND CONCLUSION:Compared with the sham group,the modified neurological severity scale scores in the model group,electroacupuncture group,low-frequency transcranial ultrasound stimulation group and combine

关 键 词：创伤性脑损伤 电针 低频经颅超声刺激 脑电信号 神经功能 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R2-03