不同方式建立伴认知障碍的耐药性癫痫模型大鼠的对比研究  

Drug-resistant epileptic rat models of cognitive impairment established by different ways:a comparative study

作　　者：李如月 任勉勉 黎辰 伍国锋 孙萍 王丽琨 Li Ruyue;Ren Mianmian;Li Chen;Wu Guofeng;Sun Ping;Wang Likun(Emergency Department,School of Clinical Medicine,Guizhou Medical University,Guiyang 550004;Emergency Department,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;Department of Emergency,Second People's Hospital of Guiyang,Guiyang 550081,China)

机构地区：[1]贵州医科大学临床医学院急诊科,贵阳550004 [2]贵州医科大学附属医院急诊科,贵阳550004 [3]贵阳市第二人民医院神经内科,贵阳550081

出　　处：《中华神经医学杂志》2024年第3期217-224,共8页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(82001380);贵州省科技计划项目(黔科合平台人才[2021]5612)。

摘　　要：目的探讨通过腹腔注射氯化锂-匹罗卡品后颅内注射匹罗卡品、卡巴胆碱两种方式建立的伴认知障碍的耐药性癫痫模型大鼠在行为学、脑电图和认知功能方面的差异。方法成年雄性SD大鼠160只,按随机数字表法选择10只作为正常对照组,50只腹腔注射氯化锂和匹罗卡品制备癫痫模型(氯化锂-匹罗卡品组),剩余100只腹腔注射氯化锂和匹罗卡品后按随机数字表法分为2组,每组50只,分别颅内注射匹罗卡品和卡巴胆碱制备癫痫模型(匹罗卡品-匹罗卡品组、匹罗卡品-卡巴胆碱组)。使用视频监控系统观察大鼠自发性癫痫发作(SRSs)次数及发作持续时间,2周后腹腔注射苯巴比妥和苯妥英钠进行耐药性筛选。采用BL-420生物信号采集处理系统记录各组大鼠脑电图波幅及癫痫波的频率。采用新事物识别实验检测大鼠对新事物的探索,采用Y迷宫自由探索实验和新异臂实验检测大鼠的空间识别记忆能力,采用Morris水迷宫实验检测大鼠的空间记忆能力。结果(1)氯化锂-匹罗卡品组大鼠存活24只(48.00%),匹罗卡品-匹罗卡品组存活25只(78.00%),匹罗卡品-卡巴胆碱组存活21只(65.62%),最终分别筛选出耐药癫痫模型大鼠7、9、8只。视频监测显示匹罗卡品-匹罗卡品组和匹罗卡品-卡巴胆碱组大鼠SRSs发作次数、发作持续时间均高于氯化锂-匹罗卡品组,差异均有统计学意义(P<0.05)。(2)匹罗卡品-匹罗卡品组、匹罗卡品-卡巴胆碱组大鼠脑电图波幅均高于氯化锂-匹罗卡品组,氯化锂-匹罗卡品组、匹罗卡品-匹罗卡品组、匹罗卡品-卡巴胆碱组大鼠脑电图癫痫波出现频率逐渐增高,差异均有统计学意义(P<0.05)。(3)正常对照组、氯化锂-匹罗卡品组、匹罗卡品-匹罗卡品组、匹罗卡品-卡巴胆碱组大鼠的鉴别指数、正确率、新异臂移动距离/总移动距离、新异臂停留时间均逐渐降低,差异均有统计学意义(P<0.05)。�Objective To investigate the behavioral,electroencephalographic,and cognitive functional differences in drug-resistant epileptic rat models of cognitive impairment prepared by intraperitoneal injection of lithium chloride-pilocarpine followed by intracranial injection of pilocarpine or carbamylcholine.Methods One hundred and sixty adult male SD rats were randomly divided into normal control group(n=10),lithium chloride-pilocarpine group(establishing epileptic rat models by intraperitoneal injection of lithium chloride-pilocarpine,n=50),pilocarpine-pilocarpine group(intracranial injection of pilocarpine after intraperitoneal injection of lithium chloride-pilocarpine,n=50)and pilocarpine-carbamylcholine group(intracranial injection of carbamylcholine after intraperitoneal injection of lithium chloride-pilocarpine,n=50).Frequency and duration of spontaneously recurrent seizures(SRSs)were observed by video monitoring system,and 2 weeks after that,phenobarbital and phenytoin sodium were injected intraperitoneally to screen drug-resistant models.Frequency and amplitude of the epileptic waves in EEG were recorded by BL-420 Bio-signal Acquisition and Processing System.Novel object recognition experiment was used to detect the novel exploration,Y-maze free exploration experiment and new and different arm experiment were used to detect the spatial recognition and memory ability,and Morris water maze experiment was used to detect the spatial memory ability.Results(1)Twenty-four rats(48.00%)survived in the lithium chloride-pilocarpine group,25(78.00%)in the pilocarpine-pilocarpine group,and 21(65.62%)in the pilocarpine-carbamylcholine group;and ultimately 7,9,and 8 drug-resistant epileptic rat models were identified,respectively;frequency and duration of SRSs in the pilocarpine-pilocarpine group and pilocarpine-carbamylcholine group were significantly higher/longer than those in the lithium chloride-pilocarpine group(P<0.05).(2)The pilocarpine-pilocarpine group and pilocarpine-carbamylcholine group had significantly higher a

关 键 词：耐药性癫痫 认知功能 匹罗卡品 卡巴胆碱 行为学 脑电图 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]