利用InDel标记鉴定典湖1号杂交种纯度  

Identification of the purity of Dianhu 1 hybrids using InDel markers

作　　者：方小雪 韦银兰 张铖锋 廖涵 胡锦彬 吴新胜 FANG Xiaoxue;WEI Yinlan;ZHANG Chengfeng;LIAO Han;HU Jinbin;WU Xinsheng(Ningbo Weimeng Seed Industry Co.,Ltd.,Ningbo 315101,Zhejiang)

机构地区：[1]宁波微萌种业有限公司,浙江宁波315101

出　　处：《浙江农业科学》2024年第5期1120-1125,共6页Journal of Zhejiang Agricultural Sciences

基　　金：宁波市“科技创新2025”重大专项(2019B10002)。

摘　　要：典湖1号是宁波微萌种业有限公司育成的青梗菜新品种,但种子纯度鉴定速度限制了该品种的推广。为实现对该品种杂交种纯度的高通量快速鉴定,在典湖1号父母本(父本EPG-194,母本EPG-168)基因组重测序的基础上开发用于纯度检测的InDel标记。试验以典湖1号及其父母本DNA样品为材料筛选到亲本带型互补、扩增条带清晰和分离速度快的引物D1-7,并用该引物检测田间典湖1号F_(1)群体(EpG-194×EpG-168群体400株,EpG-168×EpG-194群体400株)。试验中,引物D1-7的分子检测结果显示,EpG-194×EpG-168群体和EpG-168×EpG-194群体分别存在非F_(1)杂株146和32株,田间表型鉴定发现EpG-194×EpG-168群体和EpG-168×EpG-194群体分别存在非F_(1)杂株146和32株,对比后二者鉴定的800株单株结果完全一致。结果表明,InDel分子标记引物D1-7准确鉴定了典湖1号群体中混杂的母本自交种,为之后典湖1号杂交种纯度的快速鉴定奠定了基础。Dianhu 1 is a new variety of Brassica rapa L.ssp.chinensis L.bred by Ningbo Weimeng Seed Industry Co.Ltd.,but the speed of seed purity identification has limited the promotion of this variety.In order to realize high-throughput and rapid identification of the purity of hybrid seeds of this variety,InDel markers for purity detection were developed on the basis of the resequencing of the genomes of the parents of Dianhu 1(Male parent:EPG-194;Female parent:EPG-168).The DNA samples of Dianhu 1 and its parents were used to select primers D1-7 with complementary band types,clear amplification bands and fast separation speed,and the primers were used to detect the F_(1) population of Dianhu 1 in the field.The primer D1-7 was used to test the F_(1) population of Dianhu 1 in the field(400 plants for EpG-194×EpG-168 group and 400 plants EpG-168×EpG-194 group).In the experiment,the molecular detection results of primer D1-7 showed that there were 146 and 32 non-F_(1) hybrids in the EpG-194×EpG-168 group and EpG-168×EpG-194 group,and 146 and 32 non-F_(1) hybrids in the EpG-194×EpG-168 group and EpG-168×EpG-194 group were identified by field phenotyping,which were identical to the results of the 800 single plants identified by the two primers.The results showed that the InDel primers D1-7 accurately identified the mixed maternal selfed hybrids in Dianhu 1 population,which laid the foundation for the rapid identification of the purity of Dianhu 1 hybrids in the future.

关 键 词：青梗菜 纯度鉴定 INDEL 

分 类 号：S636.9[农业科学—蔬菜学]