低氧环境下生长的卵巢癌细胞株SKOV3的转录组分析  

Transcriptome Analysis of Ovarian Cancer Cell Line SKOV3 Grown in a Hypoxic Environment

作　　者：王鑫 杜珍武[2] 李晶[1] 祝贺[1] Wang Xin;Du Zhenwu;Li Jing;Zhu He(Department of obstetrics and gynecology,the Second Norman Bethune Hospital of Jilin University,ChangChun 130041;Department oforthopedics research institute,the Second Norman Bethune Hospital of Jilin University,ChangChun 130041)

机构地区：[1]吉林大学第二医院妇产科,长春130041 [2]吉林大学第二医院骨科研究所,长春130041

出　　处：《国际老年医学杂志》2024年第3期338-343,共6页International Journal of Geriatrics

基　　金：吉林省科技厅重点研发项目(20210204025YY)。

摘　　要：目的 探讨卵巢癌细胞在常氧与低氧环境培养下mRNA表达谱的差异。方法 将卵巢癌细胞株SKOV3置于低氧(2%)与常氧(21%)环境下培养,应用氧探针进行细胞乏氧状态检测,应用高通量测序对细胞内整体的mRNA进行测序,应用生物信息学分析工具对测序结果进行基因表达差异分析与功能富集分析,应用逆转录实时荧光定量PCR进行相关差异基因表达的进一步验证。结果 与常氧培养的细胞相比,在低氧环境下培养的细胞共有999个基因表达显著上调,646个基因表达显著下调。这些表达差异基因参与细胞外基质形成、细胞黏附、糖酵解、纤毛组装等生物学过程以及癌症信号、PI3K-AKT信号、RNA转运以及肿瘤胆碱代谢等信号通路。逆转录实时荧光定量PCR结果验证了低氧组的HILPDA、MT1B、CA9、MT1X与LOX-L2基因表达明显高于常氧组,而低氧组的WARS1、CHAC1、PSAT1、UPP1与DDX5基因表达明显低于常氧组。结论 本研究揭示了在不同浓度氧环境下卵巢癌细胞mRNA转录水平差异表达的相关基因及分子通路,为进一步探讨低氧环境对卵巢癌细胞生长影响的潜在分子机制提供实验基础。Objective To explore the differences in mRNA expression profiles of ovarian cancer cells cultured in normoxic and hypoxic environments.Methods The ovarian cancer SKOV3 cells were cultured separately in low oxygen(2%oxygen)and normal oxygen(21%oxygen)environments.Oxygen probes were used to detect cellular hypoxia status.The whole mRNAs expressed within cells were detected using high-through RNA sequencing,and bioinformatics analysis tools were used to analyze gene expression differences and functional enrichment of the genes,and reverse transcription quantitative real-time PCR was used to further verify the related differential gene expression.Results Compared with cells cultured under normal oxygen,cells cultured under low oxygen environment showed a total of 999 significantly upregulated gene expressions and 646 significantly downregulated gene expressions.These differentially expressed genes were involved in biological processes such as extracellular matrix formation,cell adhesion,glycolysis,ciliary assembly,as well as signaling pathways such as cancer signaling,PI3K-AKT signaling,RNA transport,and tumor choline metabolism.The reverse transcription quantitative real-time PCR results confirmed that the expression of HILPDA,MT1B,CA9,MT1X and LOX-L2 genes in the hypoxic group were significantly higher than those in the normoxic group,while the expression of WARS1,CHAC1,PSAT1,UPP1 and DDX5 genes in the hypoxic group were significantly lower than those in the normoxic group.Conclusion This study revealed the differentially expressed genes and molecular pathways of mRNA transcription levels in ovarian cancer cells under different oxygen concentrations environments,providing an experimental basis for further exploring the potential molecular mechanisms of the impact of low oxygen environments on the growth of ovarian cancer.

关 键 词：低氧 转录组 卵巢癌 SKOV3细胞 

分 类 号：R737.31[医药卫生—肿瘤]