Stigma-Specific Comparative Proteomic Analysis Reveals the Distyly Response to Self-Incompatibility in Plumbago auriculata Lam  

作　　者：Di Hu Shouli Yi Di Lin Suping Gao Ting Lei Wenji Li Tingdan Xu Songlin Jiang 

机构地区：[1]School of Fine Arts and Calligraphy,Sichuan Normal University,Chengdu,610000,China [2]Sichuan Certification and Accreditation Association,Chengdu,610000,China [3]College of Landscape Architecture,Sichuan Agricultural University,Chengdu,610000,China [4]School of Design,Chongqing Industry Polytechnic College,Chongqing,401120,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第4期681-697,共17页国际实验植物学杂志（英文）

基　　金：Scientific Research Foundation of Science&Technology Department of Sichuan Province(2021YJ0497).

摘　　要：In plants,heteromorphic self-incompatibility(HetSI)is a strategy for avoiding self-pollination and promoting outcrossing,and during this process,numerous protein-protein interaction events occur between the pistil and pollen.Previous studies in Primula and Fagopyrum that focused on HetSI systems have provided interesting insights;however,the molecular mechanism underlying HetSI remains largely unknown.In this study,we profiled the proteome of Plumbago auriculata stigmas before and after self-incompatible(SI)and self-compatible(SC)pollination.Comparative analyses were conducted by 4D-DIA(Four-dimensional data independent acquisition),a promising technology that increases the sensitivity and reduces the spectral complexity of proteomic analysis by adding a fourth dimension,ion mobility.The results revealed 33387 peptides and 5311 proteins in all samples.The pathways in which the differentially expressed proteins(DEPs)identified in the P×P(Pin style self-pollinated with pin pollen)vs.PS(Pin style)and T×T(Thrum style self-pollinated with thrum pollen)vs.TS(Thrum style)comparisons were significantly enriched were biosynthesis of secondary metabolites and pentose and glucuronate interconversions.In the P×T(Pin style cross-pollinated with thrum pollen)vs.PS and T×P(Thrum style cross-pollinated with pin pollen)vs.TS comparison,the top three pathways were biosynthesis of secondary metabolites,pentose and glucuronate interconversions,and phenylpropanoid biosynthesis.The phenylpropanoid biosynthesis,cutin,suberine and wax biosynthesis,and flavonoid biosynthesis pathways were enriched in the P×T vs.P×P comparison,and starch and sucrose metabolism,glycerophospholipid metabolism,and alpha-linolenic acid metabolism were abundant in the T×T vs.T×P comparison.The enriched pathways between PS and TS were the biosynthesis of secondary metabolites,phenylpropanoid biosynthesis,and pentose and glucuronate interconversion.Self-incompatibility protein S1(SI S1),Mitogen-activated protein kinase 3/4(MPK3/4),Mitogen-activated prote

关 键 词：Heteromorphic self-incompatibility Plumbago auriculata PROTEOMICS 4D-DIA 

分 类 号：Q94[生物学—植物学]