RNA-seq技术在虾蟹研究中的应用  

Application of RNA-seq in Shrimp and Crab Research: a Review

作　　者：谢姝敏 张惠玲 熊佳瑶 花松松 邢超凡 高焕 王攀攀 XIE Shumin;ZHANG Huiling;XIONG Jiayao;HUA Songsong;XING Chaofan;GAO Huan;WANG Panpan(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Key Laboratory of Marine Biotechnology School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Marine Resource Development Institute of Jiangsu(Lianyungang),Lianyungang 222005,China;The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室&江苏省海洋生物技术重点实验室,江苏连云港222005 [2]江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [3]江苏省海洋资源开发研究院(连云港),江苏连云港222005 [4]江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《水产学杂志》2024年第2期116-125,共10页Chinese Journal of Fisheries

基　　金：江苏省自然科学基金项目(BK20210924);江苏省高等学校基础科学面上项目(22KJB180014);江苏省产学研合作项目(BY2021366);江苏省农业农村厅“种业振兴”揭榜挂帅项目JBGS(2021)124;中国博士后科学基金面上资助项目(2022M721397)。

摘　　要：基于高通量测序技术的RNA-seq技术已被广泛应用于较高经济价值的虾蟹类动物研究中,从分子水平剖析虾蟹类生命周期中相关基因的调控功能和遗传信息。本文综述了对虾蟹类动物转录组研究现状,包括虾蟹类的生长发育、生殖繁育、环境胁迫、免疫应答、营养代谢和分子标记开发等,分析了RNA-seq技术在虾蟹类研究中面临的局限与挑战,以期为更深入开展虾蟹类分子生物学研究提供参考。RNA-seq technology based on high-throughput sequencing technology has been widely used in the research of highly economical value shrimps and crabs with.The regulatory functions of related genes in the life cycle are described at the molecular level to further consolidate the genetic information of shrimps and crabs at the molecular level.The current transcriptomes research of shrimp and crabs are discussed including growth and development,reproductive breeding,environmental stress,immune response,nutrient metabolism,and molecular marker development.On this basis,the limitations and challenges of RNA-seq technology in shrimps and crabs research are analyzed to provide reference with further molecular biology research on shrimps and crabs.

关 键 词：RNA-SEQ 虾蟹类动物 生长繁育 环境胁迫 

分 类 号：S917.4[农业科学—水产科学]