HPLC-MS/MS测定保健食品中6种大麻素  

Determination of six Cannabinoid in health food by HPLC-MS/MS

作　　者：侯巧红 张勋 赵宇 齐鹤鸣 李孟璇 步献功 董昕 李燕鹭 宋清莲 邹明强[5] 金明华[1] 胡婷婷 Hou Qiaohong;Zhang Xun;Zhao Yu;Qi Heming;Li Mengxuan;Bu Xiangong;Dong Xin;Li Yanlu;Song Qinglian;Zhou Mingqiang;Jin Minghua;Hu Tingting(Jilin University,School of Public Health,Changchun 130021,China;Changchun Customs Technology Center,Changchun 130062,China;Science and Technology Research Center of China Customs,Beijing 100026,China;Jilin Provincial Institute of Sports Science(Jilin Provincial Anti-Doping Center),Changchun 130000,China;China Academy of inspection and quarantine Sciences,Beijing 100176,China)

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]长春海关技术中心,吉林长春130062 [3]中国海关科学技术研究中心,北京100026 [4]吉林省体育科学研究所(吉林省反兴奋剂中心),吉林长春130000 [5]中国检验检疫科学研究院,北京100176

出　　处：《现代科学仪器》2024年第2期10-15,共6页Modern Scientific Instruments

基　　金：海关总署科研项目2020HK182;海南省重点研发计划项目ZDYF2021SHFZ069;吉林省科技发展计划项目(重点研发项目)20210203045SF;中国海关科学技术研究中心研课题2023HX07。

摘　　要：目的:利用高效液相色谱-串联三重四极杆质谱(HPLC-MS/MS)对保健食品中6种大麻素进行定性定量分析。方法:HLB固相萃取小柱净化,采用XBridge C18色谱柱,乙腈溶液作为有机相,10mmol·L^(-1)乙酸铵水溶液作为水相,2种离子模式同时扫描,同位素内标定量。结果:所测大麻素在1-100μg·L^(-1)浓度范围均呈现良好线性关系,相关系数(r^(2))均>0.999。方法的检出限和定量限分别为5μg·kg^(-1)和10μg·kg^(-1)。该方法稳定性好,灵敏度高,适用于常见保健食品中6种大麻素的定性定量分析。结论:本研究成功建立了保健食品中6种大麻素的定性定量分析,为保健食品品质评价、安全监管及风险防范提供技术支持。Objective:Qualitative and quantitative analysis of six cannabinoids in health food using high-performance liquid chromatography tandem triple quadrupole mass spectrometry(HPLC-MS/MS).Methods:HLB solid phase extraction column purification,using XBridge C18 chromatography column,with acetonitrile solution as the organic phase and 10 mmol·L^(-1)ammonium acetate aqueous solution as the aqueous phase.Two ions modes were scanned simultaneously and isotope internal standard quantification was performed.Results:The measured Cannabinoids between 1-100μg·L^(-1)concentration range shows a good linear relationship,and the correlation coefficients(r^(2))is greater than 0.999.The detection limit and quantification limit of the method are 5μg·kg^(-1)and 10μg·kg^(-1).The method has good stability and high sensitivity,and is suitable for qualitative and quantitative analysis of six Cannabinoid in common health products.Conclusion:This study successfully established established a qualitative and quantitative analysis of six cannabinoids in health food,providing technical support for quality evaluation,safety supervision,and risk prevention of health food.

关 键 词：大麻素 保健食品 高效液相色谱-串联质谱 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]