表面活化附着纳米银的聚醚砜抗菌膜的制备以及其在罗非鱼保鲜中的应用  

Preparation of polyethersulfone antibacterial film with nano-silver attached on surface and its application in tilapia preservation

作　　者：谭树良 赵奕辉 黄惠华 董浩 何其 吴炜亮 TAN Shuliang;ZHAO Yihui;HUANG Huihua;DONG Hao;HE Qi;WU Weiliang(School of Public Health,Southern Medical University,Guangzhou 510515,China;Institute of Light Industry and Food,Zhongkai University of Agricultural and Engineering,Guangzhou 510225,China)

机构地区：[1]南方医科大学公共卫生学院,广东广州510515 [2]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《食品与发酵工业》2024年第10期88-95,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(31901750);国家重点研究开发计划(2018YFC1602206);广东省重点研发项目(2019B020210002);广东省医学科技研究基金(20191119103719813)。

摘　　要：水产品在贮藏过程中,细菌侵染导致的腐烂变质是一个备受关注的问题。为此,该研究致力于开发一种新型抗菌包装来提高罗非鱼的保鲜效果。通过用浓硫酸将聚醚砜(polyethersulfone,PES)材料磺化并将其作为膜基质。傅里叶红外光谱仪(Fourier transform infrared spectrometer,FTIR)、热重法(thermal analysis,TG)和接触角(contact angle,CA)分析结果表明,磺化改性后提高了材料的亲水性和表面活性。在此基础上,将银纳米颗粒(silver nanoparticles,AgNPs)嵌合到膜材料中合成磺化聚醚砜-银纳米颗粒(sulfonated polyethersulfone-silver nanoparticles,SPES-AgNPs)抗菌膜。用扫描电子显微镜、原子力电子显微镜及X射线等方法分析该抑菌膜的表征,通过Ag^(+)释放实验测定其安全范围,并采用抗菌环实验测定该膜的抗菌性能。结果表明,磺化改性后的SPES材料与AgNPs结合良好,AgNPs在膜上平均尺寸约为10~100 nm。Ag^(+)释放实验显示,SPES-AgNPs膜的Ag^(+)释放量始终保持在安全范围内。SPES-AgNPs膜可以有效抑制铜绿假单胞菌、金黄色葡萄球菌、小肠结肠炎耶尔森菌、李斯特菌及大肠杆菌,其平均抑制率达到85%以上。将SPES-AgNPs膜用于罗非鱼的保鲜,所有样品在(1±0.1)℃的冷冻箱贮存18 d,通过定期对菌落总数、厌氧菌落总数、嗜冷菌落总数、总挥发性盐基氮值以及k值进行测定,并以感官评分为基础对罗非鱼样品新鲜度进行分析。结果表明,该膜能够延缓鱼肉样品保存时在化学、微生物和感官等方面的变化,可以提高罗非鱼的贮存品质并延长其保质期,是具有广阔应用前景的水产品包装材料。During the storage process of aquatic products,the deterioration caused by bacterial infection is a matter of great concern.Therefore,this study was committed to developing new antibacterial packaging for tilapia.Firstly,polyether sulfone(PES)material was sulfonated with concentrated sulfuric acid and used as a membrane substrate.The results of Fourier transform infrared spectrometer(FTIR),thermal analysis(TG),and contact angle(CA)showed that the hydrophilicity and surface activity of the material were improved after sulfonation.On this basis,silver nanoparticles(AgNPs)were intercalated into the membrane material to synthesize SPES-AgNPs antibacterial membrane.Furthermore,the characterization of the antibacterial film was analyzed by scanning electron microscope,atomic force electron microscope,and X-ray,and its safety range was determined by the Ag^(+)release test,and the antibacterial performance of the film was determined by the antibacterial ring test.Results showed that the sulfonated SPES material combined well with AgNPs,and the average size of AgNPs on the membrane was about 10-100 nm.The Ag^(+)release experiment showed that the Ag^(+)release of the SPES-AgNPs membrane was always within the safe range.SPES-AgNPs membrane could effectively inhibit Pseudomonas aeruginosa,Staphylococcus aureus,Yersinia enterocolitica,Listeria monocytogenes,and Escherichia coli,with an average inhibition rate of more than 85%.The SPES-AgNPs membranes were used for tilapia preservation,and all samples were stored in a freezer at(1±0.1)℃for 18 days by periodically determining the total number of colonies,the total number of anaerobic colonies,the total number of psychrotrophic colonies,the total volatile basic nitrogen value,as well as the k value,and analyzing the freshness of tilapia samples based on sensory scoring.The results showed that the membrane could delay the changes in chemical,microbial,and sensory aspects when fish meat samples were preserved,and could improve the storage quality and extend the shelf life of ti

关 键 词：聚醚砜 AGNPS 抗菌膜 罗非鱼 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]