镉胁迫对盐地碱蓬二型种子幼苗生长及抗逆生理的影响  

Effects of cadmium stress on dimorphic seedling growth and the stress-resistance physiology of Suaeda salsa

作　　者：崔盼盼 李彤 苏芳莉[1,2,3] CUI Panpan;LI Tong;SU Fangli(College of Water Conservancy,Shenyang Agricultural University,Shenyang 110866,China;Liaoning Panjin Wetland Ecosystem National Observation and Research Station,Shenyang 110866,China;Liaoning Shuangtai Estuary Wetland Ecosystem Research Station,Panjin 124112,China)

机构地区：[1]沈阳农业大学水利学院,沈阳110866 [2]辽宁盘锦湿地生态系统国家野外科学观测研究站,沈阳110866 [3]辽宁双台河口湿地生态系统国家定位观测研究站,辽宁盘锦124112

出　　处：《农业环境科学学报》2024年第5期991-999,共9页Journal of Agro-Environment Science

基　　金：国家重点研发计划项目(2022YFF1301004);辽宁省“兴辽英才计划”科技创新领军人才项目(XLYC2002054)。

摘　　要：为探究盐地碱蓬二型种子幼苗对Cd胁迫的适应策略,明确Cd胁迫下植物响应机理并筛选出对Cd污染盐渍土壤有修复潜力的种子类型,以辽河口湿地潮滩区盐地碱蓬(Suaeda salsa)为材料,采用土培盆栽方法对盐地碱蓬进行0、0.04、0.2、0.4、0.8 mg·kg^(-1)Cd胁迫,分析2种盐地碱蓬幼苗生长情况、不同部位Cd含量、抗氧化酶比活力和渗透调节物含量。结果表明:Cd胁迫对黑色种子幼苗株高、鲜质量和根冠比的抑制作用大于棕色种子幼苗,Cd胁迫下棕色种子幼苗根、茎、叶生物量总体高于黑色种子幼苗;盐地碱蓬棕色种子幼苗叶片SOD、POD和CAT对Cd胁迫的抗氧化响应能力强于黑色种子幼苗,渗透调节物含量随着Cd胁迫浓度的增加呈升高的趋势,且棕色种子幼苗可溶性糖和脯氨酸含量明显高于黑色种子幼苗;盐地碱蓬棕色种子幼苗器官中Cd累积含量高于黑色种子幼苗,各器官Cd的积累分配为根>叶>茎;盐地碱蓬Cd累积与渗透调节物质含量之间呈正相关(P<0.01),植物生长与抗氧化酶活性之间呈正相关(P<0.05)。研究表明,棕色种子幼苗Cd胁迫耐性强于黑色种子幼苗,盐地碱蓬棕色种子有较好的Cd污染盐土修复潜力。The adaptation strategy and response mechanism of Suaeda salsa dimorphic seedlings under Cd stress were explored,and the seed type with the greater potential to repair Cd-polluted saline soil was selected.Seedling growth,Cd accumulation in different organs,antioxidant enzyme activity,and osmotic regulator contents were examined by soil culturing S.salsa in the Liaohe Estuary wetlands with 0,0.04,0.2,0.4 mg·kg^(-1),and 0.8 mg·kg^(-1)Cd.The results were as follows.The plant height and fresh weight of black seedlings were reduced compared to those of brown seedlings,and the root,stem,and leaf biomasses of brown seedlings were larger than those of black seedlings.The antioxidative responses of SOD,POD,and CAT in brown seedlings were stronger than those in black seedlings.The osmotic regulator content increased significantly,and the soluble sugar and proline contents in brown seedlings were significantly higher than those in black seedlings.The Cd accumulation of brown seedlings was higher than that of black seedlings,and its distribution in different organs was root>leaf>stem.Cd accumulation and osmotic regulator content were positively correlated(P<0.01),as well as plant growth and antioxidant enzyme activity(P<0.05).In conclusion,brown seedlings showed stronger Cd tolerance and better ability to repair Cd-polluted saline soil than black seedlings.

关 键 词：盐地碱蓬 镉胁迫 二型种子 抗逆生理 

分 类 号：X173[环境科学与工程—环境科学]