刺激隐核虫感染对大黄鱼生理生化指标及免疫指标的影响  

Effects of Cryptocaryon irritans infection on physiological and biochemical indexes and immune indexes in large yellow croaker(Larimichthys crocea)

作　　者：胡水木 刘悦 李奕晨 田国鹏 郭心怡 林嘉琪 白玉麟 徐鹏[1,2] 周涛 HU Shuimu;LIU Yue;LI Yichen;TIAN Guopeng;GUO Xinyi;LIN Jiaqi;BAI Yulin;XU Peng;ZHOU Tao(State Key Laboratory of Mariculture Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen 361102,China;Fujian Province Key Laboratory of Marine Biological Genetic Breeding,College of Ocean and Earth Sciences,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学海洋与地球学院,海水养殖生物育种全国重点实验室,福建厦门361102 [2]厦门大学海洋与地球学院,福建省海洋经济生物遗传育种重点实验室,福建厦门361102

出　　处：《水产学报》2024年第5期83-91,共9页Journal of Fisheries of China

基　　金：国家自然科学基金(U21A20264);国家重点研发计划(2022YFD2401002);福建省种业创新与产业化工程项目(2021FJSCZY01);厦门大学大学生创新创业训练计划项目(S202210384455)。

摘　　要：刺激隐核虫感染能够导致海水鱼类感染“白点病”,而大黄鱼是受“白点病”影响最严重的海水养殖鱼类。为了探究刺激隐核虫感染对大黄鱼生理生化指标及免疫指标的影响,本研究利用刺激隐核虫人工感染大黄鱼,分别在感染后0、12、24、48和72 h采集血液、肝脏、脾脏、肠道、鳃、头肾和皮肤组织,并检测血清皮质酮、皮质醇以及肝脏谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量等指标的变化。同时使用实时荧光定量PCR(qRT-PCR)实验检测肝脏、脾脏、肠道、鳃、头肾和皮肤组织中TNF-α、IL-8和IL-1β基因的表达量。结果显示,在大黄鱼感染刺激隐核虫后的0~72 h内,实验组大黄鱼表现出刺激隐核虫病的发病症状;血清皮质醇和皮质酮含量显著增加;肝脏GSH-Px活性极显著降低;肝脏SOD活性极显著增加;肝脏MDA含量在0~12 h内急剧增加并达到峰值,随后含量缓慢降低;各组织中的TNF-α、IL-8和IL-1β的表达量均有不同程度的上调,在鳃、头肾、肝脏和皮肤中上调最为显著。研究表明,刺激隐核虫感染后有明显变化的皮质类激素含量和氧化应激指标能够反映大黄鱼的感染程度,有助于进一步辅助抗刺激隐核虫表型测量的优化。本研究可为深入理解刺激隐核虫感染后大黄鱼生理生化及免疫指标的动态变化提供基础,为后续的机制解析和品种选育工作提供参考。The large yellow croaker(Larimichthys crocea)is the marine culture fish most affected by white spot disease,with the highest frequency of C.irritans outbreaks in China.After years of efforts from scholars at home and abroad,many research progresses have been made in C.irritans propagation,pathogen pathology and vaccine research and development,but there are fewer studies on the physiological,biochemical and immune indexes of L.crocea infected with C.irritans.In order to understand the changes in various physiological and immune indicators of L.crocea after C.irritans infection,in this study,we selected 300 experimental fish for artificial C.irritans infection experiments at the peak of hatching of C.irritans cyst.The experimental fish were randomly assigned to three experimental groups and one control group.Blood,liver,spleen,intestines,gills,head kidney,and skin tissues were collected at 0,12,24,48,and 72 h after infection to monitor changes in serum cortisol and corticosterone levels,liver glutathione peroxidase(GSH-Px)activity,superoxide dismutase(SOD)activity and malondialdehyde(MDA)level.Transcription patterns of TNF-α,IL-8 and IL-1βin liver,spleen,intestine,gill,head kidney,and skin tissues were detected by quantitative real-time PCR experiments.During the 3 days after infection,the serum cortisol and corticosterone level increased significantly and gradually from 0 h to 72 h after C.irritans infection(P<0.05);the GSH-Px activity in the liver showed a highly significant decreasing trend(P<0.001);the SOD activity in the liver showed an overall upward trend except for a decrease at 48 h(P<0.001);the MDA level in the liver increased sharply within 12 h,and began to decrease after reaching the peak(P<0.05);the expression of TNF-α,IL-8 and IL-1βin various tissues were upregulated to varied degrees,with those in the gills,head kindey,liver,and skin being the most obvious,which indicated these organs may play important roles in the immune response against C.irritans.These results suggested that corticosteroid

关 键 词：大黄鱼 刺激隐核虫 生理生化指标 免疫指标 抗虫性状评价 

分 类 号：S941.5[农业科学—水产养殖]