紫外光固化纳米纤维素/聚乙二醇二丙烯酸酯体系固定化脂肪酶的研究  

Study on Immobilization of Lipase on UV Curing Nanocellulose/PEGDA Hydrogel

作　　者：陈玟锦 李静[1] 唐爱民[1] 刘旺玉[2] 林影[3] CHEN Wenjin;LI Jing;TANG Aimin;LIU Wangyu;LIN Ying(State Key Laboratory of Pulp and Paper Engineering South China University of Technology,Guangzhou 510640,China;School of Mechanical and Automotive Engineering South China University of Technology,Guangzhou 510640,China;School of Biology and Biological Engineering South China University of Technology,Guangzhou 510640,China)

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]华南理工大学机械科学与工程学院,广东广州510640 [3]华南理工大学生物科学与工程学院,广东广州510640

出　　处：《造纸科学与技术》2024年第2期11-16,38,共7页Paper Science & Technology

基　　金：国家自然科学基金项目(51875214)。

摘　　要：以羧基改性的纳米纤维素(TOCNF)和聚乙二醇二丙烯酸酯(PEGDA)为载体,通过紫外光固化实现了脂肪酶的原位包埋固定化,建立了紫外光固化TOCNF/PEGDA固定化酶的体系。研究了制备条件对固定化的影响,确定了制备最佳条件:PEGDA为15 wt%,TOCNF为1.0 wt%,光固化时间为60 s,固定化酶表现出95.90%的负载率和1970 U/g的酶活力;研究了固定化酶的酶活稳定性,固定化酶表现出3.10%的酶泄露率,经过20天储存后干燥的固定化酶保留89.91%的相对活性,经过8次重复水解操作后保留84.76%的相对活性。A system of UV-cured carboxyl-modified nanocellulose(TOCNF)/poly(ethylene glycol)diacrylate(PEGDA)immobilization was established for in situ embedding and immobilization of lipase.The optimal preparation conditions of 15 wt%PEGDA,1.0 w t%TOCNF,and 60 s UV curing time were investigated,and the immobilized lipase had a loading rate of 95.90%and an enzyme activity of 1970 U/g.The stability of the immobilized lipase was studied.The immobilized lipase exhibited an enzyme leakage rate of 3.10%and retained 89.91%relative activity after 20 days of storage for dried immobilized lipase and 84.76%relative activity after 8 repetitions of hydrolysis of butyl p-nitrophenol(pNPB)operation.

关 键 词：脂肪酶 固定化 纳米纤维素 紫外光固化 聚乙二醇二丙烯酸酯 

分 类 号：TB383[一般工业技术—材料科学与工程]