雷帕霉素上调人脐静脉内皮细胞自噬活性抑制细胞增殖  

Rapamycin upregulates autophagy inhibits cell proliferation in human umbilical vein endothelial cells

作　　者：王雅雯[1] 程亚楠[1] 杨宾 苏碧昊 徐普[1] Wang Yawen;Cheng Yanan;Yang Bin;Su Bihao;Xu Pu(Dept of Stomatology,Haikou Hospital,Hainan Stomatological Center,Central South University Xiangya School of Medicine Affiliated Haikou Hospital,Haikou 570208)

机构地区：[1]中南大学湘雅医学院附属海口医院口腔中心·海南省口腔医学中心口腔综合科,海口570208

出　　处：《安徽医科大学学报》2024年第4期605-610,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82060194);海南省重点研发计划项目(编号:ZDYF2022SHFZ119);海南省自然科学基金高层次人才项目(编号:821RC727、821RC725)。

摘　　要：目的探讨人脐静脉内皮细胞(HUVECs)自噬激活对细胞增殖的影响。方法使用雷帕霉素(Rapa)处理HUVECs,Western blot法检测微管相关蛋白1轻链3(LC3)、Beclin 1和unc-51样激酶1(ULK1)的表达,透射电镜(TEM)检测自噬小体,丹酰尸胺染色(MDC)检测自噬荧光;CCK-8法和EdU法检测自噬激活对细胞增殖的影响;血管形成实验检测成管能力。结果Rapa处理后,与对照组相比,LC3、Beclin 1和ULK1表达增强,实验组绿色自噬荧光表达强于对照组,TEM可见自噬小体;CCK-8和EdU结果显示,与对照组相比,实验组细胞自噬活化后细胞增殖能力减弱,成管能力降低。结论在一定时间内,Rapa上调HUVECs自噬活性抑制细胞增殖。Objective To investigate the effect of autophagy activation on cell proliferation in human umbilical vein endothelial cells(HUVECs).Methods HUVECs were treated with rapamycin(Rapa).Western blot assay was performed to examine the expression of protein of microtubule associated protein 1 light chain 3(LC3),Beclin 1 and unc-51-like kinase 1(ULK1).Autophagosomes were detected by transmission electron microscopy(TEM),and autophagy fluorescence was detected by monodansylcadaverine staining(MDC)assay.The effect of autophagy activation on cell proliferation was assessed by CCK-8 assay and EdU assay.Vascular formation experiments were used to detect vasculogenic ability.Results After Rapa treatment,LC3,Beclin1 and ULK1 expressions were enhanced,while the green autophagy fluorescence expression in the experimental group was stronger than that in the control group,and autophagosomes were visible by TEM;CCK-8 and EdU results showed that compared with the control group,the cell proliferation ability was weakened and tubes formation ability was reduced after the activation of autophagy in experimental cells.Conclusion Rapa upregulates autophagy activity in HUVECs to inhibit cell proliferation under certain time.

关 键 词：雷帕霉素 人脐静脉内皮细胞 自噬 自噬活性 自噬蛋白 细胞增殖 

分 类 号：R783.4[医药卫生—口腔医学]