1株中肋骨条藻溶藻菌的培养优化及其溶藻物质特性分析  

Cultivation Optimization and Algicidal Substances Characteristics of an Algicidal Bacterium on Skeletonema costatum

作　　者：潘知顺 张凯洋 蒋贤龙 乔冰清 丁文娟 徐晴倩 梁蜜 何梦岚 PAN Zhishun;ZHANG Kaiyang;JIANG Xianlong(School of Marine Science and Technology of Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院,浙江舟山316022

出　　处：《浙江海洋大学学报（自然科学版）》2024年第2期159-167,共9页Journal of Zhejiang Ocean University：Natural Science

基　　金：国家级大学生创新创业训练计划(202110340059);浙江省基础公益研究计划(LGG21D060001)。

摘　　要：中肋骨条藻是舟山近岸海域的赤潮优势种之一,前期研究筛得1株溶藻菌,为芽孢杆菌属,对中肋骨条藻有明显抑制作用,但溶藻物质成分尚不明确。采用单因子试验获得该菌最优培养条件;采用单因子试验和正交试验分析溶藻物质稳定性;最后通过酶解试验和高效液相色谱初步探索溶藻物质的可能成分。在实验条件范围内,pH 8.0,温度25℃,盐度34是溶藻菌的最优培养条件。溶藻物质具有耐酸碱,耐高温、耐高盐、抗紫外的特点,受pH影响最大,其次是盐度和温度,最后是紫外光。推测溶藻物质可能是一种耐高温的非蛋白质物质,在碱性条件下的裂解活性较高,溶藻活性可能与RNA相关。通过改善培养条件可以扩增溶藻菌生物量,溶藻物质对环境具有一定的耐受性且成分与核糖核酸相关,为溶藻物质成分提供了新的可能。Harmful red tide is a harmful marine ecological phenomenon,and Skeletonema costatum is one of the algae that cause harmful algae blooms.In the previous study,a strain of algicidal bacteria was screened,which was a species of Bacillus(Bacillus sp.2-1-2),it has obvious inhibitory effect on the S.costatum,but the content of this algicidal bacteria is very small in natural water,and the specific inhibition mechanism has not been clear.To explore the optimal cultivation conditions,stability and composition of algicidal bacteria,and to lay a foundation for the actual water application of algicidal bacteria.First,a single factor test was used to analyze the effects of initial pH,temperature and salinity on the growth and effect of algicidal bacteria,and the optimal culture conditions were obtained.Secondly,single-factor test and orthogonal test were used to analyze the effects of pH,temperature,salinity and ultraviolet light on the stability of algicidal substances,Finally,the possible components of algicidal substances were preliminarily explored by enzymatic hydrolysis test and HPLC.pH 8.0,temperature 25℃,salinity 34 were the optimal culture conditions for algicidal bacteria within the experimental conditions.Algicidal substances have the characteristics of acid and alkali resistance,high temperature resistance,high salt resistance,and ultraviolet resistance,and are most affected by pH,followed by salinity and temperature,and finally ultraviolet light.It is speculated that the algicidal substance may be a non-protein substance with high temperature resistance and high algicidal activity under alkaline conditions,and the algicidal activity may be related to RNA.By improving the culture conditions,the biomass of algicidal bacteria can be amplified,and the algicidal substances have certain tolerance to the environment and their components are related to RNA,which provides a new possibility for the active components of algicidal substances.

关 键 词：溶藻菌 优化培养 溶藻物质 成分鉴定 

分 类 号：X17[环境科学与工程—环境科学] X55