摘叶处理对‘红地球’葡萄品质及部分类黄酮合成基因表达的影响  

Effect of Leaf Removal on Fruit Quality and Expression of Flavonoid Synthesis-related Genes in‘Red Globe’Grapevine

作　　者：杨博唯 曾茂宇 张振文[1,2] 谢沙 YANG Bowei;ZENG Maoyu;ZHANG Zhenwen;XIE Sha(College of Enology,Northwest A&F University,Yangling 712100,China;Shaanxi Engineering Research Center of Grape and Wine,Yangling 712100,China)

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100

出　　处：《中外葡萄与葡萄酒》2024年第3期53-60,共8页Sino-Overseas Grapevine & Wine

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-29-zp-6);宁夏回族自治区科技重大专项(2023BCF01001)。

摘　　要：以‘红地球’葡萄为材料,分别在果实转色始期和转色末期进行1、2、4片摘叶处理,对不同摘叶处理的果实理化指标、果皮酚类物质含量进行检测分析,并将这些品质指标进行主成分分析和综合评价;同时选择得分较高的2个处理,对其类黄酮合成相关基因和转录因子进行实时荧光定量PCR分析,并对酚类物质含量与类黄酮合成基因进行相关性分析。结果表明,不同摘叶处理对葡萄理化指标、酚类物质含量的影响存在差异,多数摘叶处理提升了果实的糖含量,增加了果皮酚类物质含量,上调了类黄酮相关基因和转录因子的相对表达量,CHS3、DFR1、FAOMT、CHI2和GST4基因与酚类物质的含量关系密切。通过主成分分析得出,在‘红地球’葡萄转色末期从结果枝基部摘除1片叶处理,所得果实的综合品质最佳。Taking‘Red Globe’grapevine as materials,one,two,four leaves were picked at the beginning and end of fruit veraison,respectively.The physicochemical indexes and phenolic substances content of fruits under different leaf removal treatments were detected and analyzed,and these quality indexes were analyzed by principal component analysis and comprehensive evaluation.At the same time,the two better treatments were selected for real-time fluorescence quantitative analysis of flavonoid synthesis-related genes and transcription factors,and the correlation between phenolic substances content and flavonoid synthesis genes was analyzed.The results showed that different leaf removal treatments had different effects on the physicochemical indexes and phenolic substances content of grapes.Most leaf removal treatments increased the sugar content of fruits,increased the phenolic substances content of peel,and up-regulated the relative expression levels of related genes and transcription factors.The genes of CHS3,DFR 1,FAOMT,CHI 2 and GST4 were closely related to the phenolic substances content.The results of principal component analysis showed that one leaf was removed from the base of fruit branch at the end of veraison of‘Red Globe’grapes,and the comprehensive quality of the fruit was the best.

关 键 词：红地球 鲜食葡萄 品质 酚类物质 基因 

分 类 号：S663.1[农业科学—果树学]