应用微卫星位点分析3个封闭群比格犬群体遗传结构  

Genetic Structure Analysis of Three Outbred Beagle Dog Populations Using Microsatellite Markers

作　　者：蒋辉 李银银[1] 李长龙[1] 霍学云[1] 郭萌 陈振文[1] 李胜利 吕建祎[1] 杜小燕[1] JIANG Hui;LI Yinyin;LI Changlong;HUO Xueyun;GUO Meng;CHEN Zhenwen;LI Shengli;LYU Jianyi;DU Xiaoyan(School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China;Department of Laboratory Animal,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学基础医学院,北京100069 [2]首都医科大学实验动物部,北京100069

出　　处：《实验动物科学》2024年第2期6-14,共9页Laboratory Animal Science

基　　金：国家科技重点研发计划(2017YFD0501602);国家重点研发计划青年科学家项目(2021YFF0703200)。

摘　　要：目的采用比格犬微卫星位点对多地小型比格犬群体进行遗传检测和群体结构分析,并评价所选位点是否适用于封闭群比格犬遗传质量控制。方法选取前期验证的微卫星位点20个,采用其已优化的PCR条件,对来自3个公司(西安、北京和江苏)的小型比格犬群体进行PCR扩增和STR扫描,经基因分型后计算群体遗传参数并进行群体遗传结构分析。结果西安、北京和江苏三地小型比格犬群体在20个微卫星位点的平均有效等位基因数分别为5.45、6.80、6.10,平均杂合度为0.5852、0.6538、0.6631,香农指数为1.1774、1.3844、1.3498,多态性信息含量分别为0.5397、0.6147、0.6181,说明三群体遗传多态性信息丰富,群体遗传多样性高。所选20个微卫星位点既能反映群体多态性,又能体现各群体的特征。结论3个比格犬群体的多态性较高,达到了封闭群要求。所选20个微卫星位点比较适合用于比格犬遗传质量检测。Objective To analyze the genetic structure of outbred populations of small Beagle dogs from variant locations using the selected microsatellite loci,and evaluate if these markers are suitable for genetic detection of the outbred Beagle dog.Method A total of 20 microsatellite markers that has been verified were used to amplifying and STR scanning the sample from 3 companies(Xi’an,Beijing and Jiangsu)outbred populations of small beagle dogs by optimized PCR conditions.After genotyping,the genetic indexes were calculated and genetic population structure were analyzed.Result The number of average effective allele of Xi’an,Beijing and Jiangsu outbred populations of small beagle dogs exhibited with 5.45,6.80,6.10,respectively;average heterozygosity was 0.5852,0.6538,0.6631,respectively,Shannon’s information index was 1.1774,1.3844,1.3498 and PIC was 0.5397,0.6147,0.6181,respectively.It showed that 3 examined population displayed high and abundant genetic population polymorphism and high genetic diversity.The 20 microsatellite loci we used could reflect the general genetic polymorphism character of each population,as well as its unique feature.Conclusion Three outbred populations of small beagle dogs harbor comparably high polymorphism and match the demand of outbred population animal.The set of 20 microsatellite loci we optimized could be adapted for genetically monitoring outbred Beagle dogs.

关 键 词：比格犬 微卫星DNA 群体遗传结构 遗传信息 遗传检测 

分 类 号：Q95-3[生物学—动物学]