血清miR-30a-3p联合H.pylori抗体检测对老年人群早期胃癌的诊断价值  

Analysis of the diagnostic value of serum miR-30a-3p combined with Helicobacter pylori antibody detection for early gastric cancer in the elderly

作　　者：汪甜 刘满华 王雅洁 余愿 曹卉 WANG Tian;LIU Manhua;WANG Yajie(Department of Geriatrics,Xianning Central Hospital,Hubei,Xianning 437000,China)

机构地区：[1]湖北省咸宁市中心医院老年病科,437000 [2]华中科技大学同济医学院附属协和医院消化内科 [3]华中科技大学同济医学院附属梨园医院消化内科

出　　处：《河北医药》2024年第10期1468-1471,1477,共5页Hebei Medical Journal

基　　金：湖北省自然科学基金(编号:WJ2021M248)。

摘　　要：目的探究血清微小RNA-30a-3p(miR-30a-3p)联合幽门螺杆菌(H.pylori)抗体检测对老年人群早期胃癌的诊断价值。方法选取2020年5月至2022年12月进行胃镜检查的87例老年人群胃部疾病患者作为研究对象,根据病理学检查结果将患者分为慢性萎缩性胃炎(CAG)组(n=39)、早期胃癌组(n=27)和进展期胃癌组(n=21);同期选取33例健康体检者作为正常对照组。采用荧光定量PCR法(qRT-PCR)和化学发光法分别检测血清miR-30a-3p表达水平和H.pylori抗体;比较4组血清miR-30a-3p水平及H.pylori抗体阳性率;比较H.pylori抗体阳性与阴性者血清miR-30a-3p水平;Logistic回归分析影响老年人群早期胃癌的相关因素;受试者工作特征(ROC)曲线分析血清miR-30a-3p、H.pylori抗体对老年人群早期胃癌的诊断价值。结果早期胃癌组和进展期胃癌组血清miR-30a-3p表达水平均显著低于CAG组、对照组,早期胃癌组、进展期胃癌组和CAG组H.pylori抗体阳性率均显著高于对照组(P<0.05);纳入的87例胃部疾病患者和33例健康体检者中,H.pylori抗体阳性者血清miR-30a-3p显著低于H.pylori抗体阴性者(P<0.05);行Logistic回归分析结果显示,血清miR-30a-3p、H.pylori抗体阳性为影响老年人群发生早期胃癌的相关因素(P<0.05);血清miR-30a-3p联合H.pylori抗体诊断老年人群早期胃癌的曲线下面积(AUC)为0.957,优于血清miR-30a-3p、H.pylori抗体各自单独诊断(Z二者联合-miR-30a-3P=2.680、Z二者联合-H.pylori抗体=4.577,P=0.007、P<0.001),联合诊断的灵敏度和特异度分别为85.19%、96.67%。结论血清miR-30a-3p在老年人群早期胃癌患者中呈低表达,H.pylori抗体阳性率较高,二者联合检测对老年人群早期胃癌具有较高的诊断价值。Objective To explore the diagnostic value of serum microRNA-30a-3p(miR-30a-3p)combined with Helicobacter pylori(H.pylori)antibody detection for early gastric cancer in the elderly.Methods From May 2020 to December 2022,87 elderly patients with gastric diseases who underwent gastroscopy in our hospital were collected as the study subjects.According to the pathological examination results,patients were grouped into chronic atrophic gastritis(CAG)group(n=39),early gastric cancer group(n=27)and advanced gastric cancer group(n=21).During the same period,33 healthy individuals were regarded as the control group.Fluorescence quantitative PCR(qRT-PCR)and chemiluminescence were applied to detect serum miR-30a-3p expression level and H.pylori antibodies,respectively.Serum miR-30a-3p and the positive rate of H.pylori antibodies were compared.Serum miR-30a-3p level was compared between patients with positive versus negative H.pylori antibody.Logistic regression was applied to analyze the risk factors for early gastric cancer in the elderly.Receiver operating characteristic(ROC)curves were plotted to analyze the diagnostic value of serum miR-30a-3p and H.pylori antibodies for early gastric cancer in the elderly.Results The serum miR-30a-3p level in the early gastric cancer group and advanced gastric cancer group was significantly lower than that in the CAG group and control group(P<0.05).The positive rate of H.pylori antibody in the early gastric cancer group,advanced gastric cancer group,and CAG group was significantly higher than that in the control group(P<0.05).Among the 87 patients with gastric diseases and 33 healthy individuals who underwent physical examination,serum miR-30a-3p level in individuals with positive H.pylori antibody was significantly lower than that in those with negative H.pylori antibody(P<0.05).Logistic regression analysis showed that serum miR-30a-3p and positive H.pylori antibodies were risk factors for early gastric cancer in the elderly(P<0.05).The area under the curve(AUC)of serum miR-30a-3p comb

关 键 词：微小30a-3p 幽门螺杆菌抗体 老年人群早期胃癌 诊断价值 

分 类 号：R735.2[医药卫生—肿瘤]