反相高效液相色谱法同时测定清脑降压片中黄芩苷、阿魏酸和藁本内酯的含量  

Simultaneous Determination of Baicalin,Ferulic acid and Ligustilide in Qingnao Jiangya Tablet by Reversed-phase High-performance Liquid Chromatography

作　　者：郭宝凤 高寒 塔娜 贾静涛 GUO Baofeng;GAO Han;TA Na;JIA Jingtao(Inner Mongolia Autonomous Region Drug Inspection and Research Institute/Inner Mongolia Autonomous Region China-Mongolia Medicine Standard Research Key Laboratory,Hohhot 010010,China;Inner Mongolia Autonomous Region Medical Device Inspection and Testing Research Institute,Hohhot 010041,China)

机构地区：[1]内蒙古自治区药品检验研究院,内蒙古自治区中蒙药标准研究重点实验室,呼和浩特010010 [2]内蒙古自治区医疗器械检验检测研究院,呼和浩特010010

出　　处：《中国民族民间医药》2024年第9期46-50,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立测定清脑降压片中黄芩苷、阿魏酸和藁本内酯含量的反相高效液相色谱法。方法:色谱柱采用ODS-C 18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%磷酸水梯度洗脱;黄芩苷、阿魏酸、藁本内酯检测波长为326 nm;流速为1.0 mL·min-1;柱温35℃。结果:黄芩苷在221.62~3324.30 ng范围内与峰面积线性关系良好(Y=0.0654X-0.0021,γ=1.0000)。平均加样回收率为98.6%,RSD为1.42%(n=6),阿魏酸在20.07~401.40 ng范围内与峰面积线性关系良好(Y=1.0469X-0.9536,γ=1.0000)。平均加样回收率为97.8%,RSD为1.05%(n=6)。藁本内酯在20.13~402.60 ng范围内与峰面积线性关系良好(Y=0.6333X-0.6877,γ=1.0000)。平均加样回收率为98.2%,RSD为1.42%(n=6)。结论:本方法可行、准确、重复性好,可作为清脑降压片中黄芩苷、阿魏酸和藁本内酯的质量控制方法。Objective To establish a method for determination of ferulic acid and ligustilide in Qingnao Jiangya tablets.Method Use high performance liquid chromatography(HPLC),the column is C 18 column,the mobile phase was eluted with acetonitrile-0.5%phosphoric acid gradient.Baicalin、Ferulic acid and ligustilide were detected at a wavelength of 326 nm.The flow rate was 1.0 mL/min and the column temperature was 35℃.Results Baicalin had a good linear relationship with peak area in the range of 221.62~3324.30ng(Y=0.0654X-0.0021,γ=1.0000).The average sample recovery was 98.6%,and the RSD was 1.42%(n=6).There was a good linear relationship between the concentration of ferulic acid and the peak area in the range of 20.07-401.40ng(Y=1.0469X-0.9536,γ=1.0000).The average recovery was 97.8%and the RSD was 1.05%(n=6).and there was a good linear relationship between the concentration of ligustilide and the peak area in the range of 20.13-402.60 ng.(Y=0.6333X-0.6877,γ=1.0000),The average recovery was 98.2%and the RSD was 1.42%(n=6).Conclusion The method is accurate,accurate and reproducible,and it can be used as a method for the determination of Baicalin、Ferulic acid and Ligustilide in Qingnao Jiangya tablets.

关 键 词：清脑降压片 反相高效液相色谱法 黄芩苷 阿魏酸 藁本内酯 

分 类 号：R917[医药卫生—药物分析学]