贯叶金丝桃化学成分的分离与鉴定  

Isolation and identification of chemical constituents from Hypericum perforatumn L.

作　　者：胡诗雨 张梦雪 方容 杨金花 雷亮 宋红萍 毕晓宾[3] HU Shiyu;ZHANG Mengxue;FANG Rong;YANG Jinhua;LEI Liang;SONG Hongping;BI Xiaobin(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430061,China;Wuhan Fourth Hospital,Wuhan 430033,China;School of Pharmacy,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430061 [2]武汉市第四医院,湖北武汉430033 [3]华中科技大学药学院,湖北武汉430030

出　　处：《沈阳药科大学学报》2024年第5期551-556,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金青年项目(32000282);武汉市卫健委面上项目(WZ21C40);武汉市卫健委青年项目(WZ22Q21)。

摘　　要：目的研究藤黄科(Guttiferae)金丝桃属(Hypericum)贯叶金丝桃(Hypericum perforatum L.)的化学成分。方法采用正相硅胶色谱、反相ODS色谱、凝胶色谱及高效液相色谱等多种色谱方法对贯叶金丝桃体积分数95%乙醇提取物进行分离纯化。利用紫外、红外、核磁共振光谱,高分辨质谱及X-ray单晶衍射法对化合物的结构进行鉴定。结果从贯叶金丝桃中分离鉴定8个化合物,分别为perforxanthone A(1)、8-hydroxy-1,2,3-trimethoxyxanthone(2)、绵马酚D(3)、(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(4)、ent-4(15)-eudesmene-1β,6α-diol(5)、methyl-(E)-8-oxooctadec-9-enoate(6)、模绕酮酸(7)、白桦脂酸(8)。结论化合物1为新的氧杂蒽酮类化合物,化合物2-7为首次从该植物中分离得到。Objective To study the chemical composition of Hypericum perforatum L.Methods Various chromatographic methods such as normal-phase silica gel chromatography,reversed-phase silica gel chromatography,high performance liquid chromatography and gel chromatography were used to separate and purify the 95%EtOH extract of this plant.Structures of the isolated compounds were identified by NMR,UV,and IR spectral,high resolution mass spectrum(HRM),and X-ray single crystal diffraction.Results Eight compounds were isolated and identified from this plant,namely:perforxanthone A(1),8-hydroxy-1,2,3-trimethoxyxanthone(2),aspidinol D(3),(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(4),ent-4(15)-eudesmene-1β,6α-diol(5),methyl-(E)-8-oxooctadec-9-enoate(6),moronic acid(7),betulinic acid(8).Conclusion Compound 1 is a new compound.Compounds 2-7 are isolated from this plant for the first time.

关 键 词：贯叶金丝桃 提取分离 结构鉴定 

分 类 号：R914[医药卫生—药物化学]