7种miRNAs联合检测用于原发性肝癌的诊断价值  

Diagnostic value of 7 miRNAs combined test for primary liver cancer

作　　者：邓洲 刘莉[1] 何雨倩 陈悦[1] DENG Zhou;LIU Li;HE Yu-Qian;CHEN Yue(Department of Infectious Diseases,Taihe Hospital Affiliated to Hubei University of Medicine(Shiyan Hubei,442000),China)

机构地区：[1]湖北医药学院附属太和医院感染科,湖北十堰442000

出　　处：《中西医结合肝病杂志》2024年第5期405-408,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:分析7种微小核糖核酸(miRNAs)联合检测用于原发性肝癌诊断的临床应用价值。方法:采用荧光PCR技术检测原发性肝癌组、转移性肝癌组、肝硬化组共131例患者的7种miRNAs的表达水平,比较7种miRNAs在3组患者中的阳性率;分析7种miRNAs在不同病理类型肝癌中的诊断价值;比较7种miRNAs在甲胎蛋白(AFP)阴性以及AFP和异常凝血酶原(PIVKA-Ⅱ)均阴性的肝细胞癌(HCC)患者中的阳性率;分析AFP、7种miRNA以及AFP+7种miRNAs用于HCC诊断的敏感度、特异度,并运用ROC曲线评价三者的诊断效能。结果:原发性肝癌组患者7种miRNAs阳性率显著高于转移性肝癌组(χ2=13.388,P<0.01)和肝硬化组(χ2=30.827,P<0.01);在不同细胞类型的肝癌中,HCC组患者7种miRNAs的阳性率显著高于胆管细胞癌(ICC)组(χ2=6.805,P<0.01);AFP、PIVKA-Ⅱ以及7种miRNAs在HCC组患者中阳性率分别为60.71%、64.28%和75.00%;AFP阴性以及AFP和PIVKA-Ⅱ均阴性的HCC患者中,7种miRNAs联合检测阳性率分别为81.8%和80.0%;AFP、7种miRNAs以及AFP+7种miRNAs联合检测对于HCC患者诊断灵敏度分别为60.71%、75.00%和89.29%,特异度分别为81.33%、85.33%和78.67%;AFP、7种miRNAs以及AFP+7种miRNAs联合用于HCC诊断的AUC(ROC曲线下面积)分别为0.708、0.839和0.864。结论:7种miRNAs在HCC患者中具有较高的阳性率,在AFP阴性的肝癌中也具有较高的阳性率;AFP+7种miRNAs联合检测可以显著提高HCC诊断的阳性率。Objective:To analyze the clinical application value of combined detection of seven miRNAs in the diagnosis of primary liver cancer.Methods:Real-time PCR was used to detect the expression levels of seven miRNAs in 131 patients with primary liver cancer,metastatic liver cancer,and liver cirrhosis,and the positive rates of seven miRNAs in the three groups were compared.The diagnostic value of seven miRNAs in different pathological types of liver cancer was analyzed.To compare the positive rates of seven miRNAs in alpha-fetoprotein(AFP)negative and both AFP and abnormal prothrombin(PIVKA-Ⅱ)negative hepatocellular carcinoma(HCC)patients.The sensitivity and specificity of AFP,seven miRNAs and AFP+seven miRNAs in the diagnosis of HCC were analyzed,and the ROC curve was used to evaluate the diagnostic efficacy of the three.Results:The positive rate of seven miRNAs in primary liver cancer group was significantly higher than that in metastatic liver cancer group(χ^(2)=13.388,P<0.01)and cirrhosis group(χ^(2)=30.827,P<0.01).In different cell types of HCC,the positive rate of seven miRNAs in HCC group was significantly higher than that in ICC group,and the difference was statistically significant(HCC group vs ICC groupχ2=6.805,P<0.01).The positive rates of AFP,PIVKA-Ⅱand seven miRNAs in HCC patients were 60.71%,64.28%and 75%,respectively.In HCC patients with negative AFP or negative AFP and PIVKA-Ⅱ,the positive rates of combined detection of seven miRNAs were 81.8%and 80.0%,respectively.The sensitivity of AFP,seven miRNAs and AFP+seven miRNAs in the diagnosis of HCC patients were 60.71%,75.00%and 89.29%,respectively,and the specificity were 81.33%,85.33%and 78.67%,respectively.The AUC(area under the ROC curve)of AFP,seven miRNAs and AFP+seven miRNAs in the diagnosis of HCC were 0.708,0.839 and 0.864,respectively.Conclusion:The seven miRNAs have high positive expression rates in HCC patients and AFP negative HCC.The combined detection of AFP and seven miRNAs can significantly improve the positive rate of HCC diagnosis

关 键 词：肝细胞癌 微小核糖核酸 甲胎蛋白 异常凝血酶原 

分 类 号：R739.5[医药卫生—肿瘤]