草地早熟禾胚胎发生标记基因BBM的鉴定及系统进化分析  

Identification and phylogenetic analysis of the em⁃bryogenic marker gene BBM in kentucky bluegrass(Poa pratensis)

作　　者：余江弟 苗佳敏 李玉珠[1] 马晖玲[1] YU Jiang-di;MIAO Jia-min;LI Yu-zhu;MA Hui-ling(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2024年第2期38-48,共11页Grassland and Turf

基　　金：甘肃省高等学校创新基金项目(2021B-127);甘肃农业大学科技创新基金(青年导师扶持基金)(GAU-QDFC-2021-02)。

摘　　要：【目的】利用生物信息学方法,鉴定草地早熟禾(Poa pratensis)胚胎发生标记基因PpBBM,并通过保守基序比对和进化树分析,推测可能具有孤雌生殖功能的PpBBM基因。【方法】基于草地早熟禾转录组数据库信息,检索PpBBM基因。通过生物信息学软件分析PpBBMs蛋白理化特性,比较不同PpBBMs蛋白的系统进化关系和保守基序差异。【结果】共获得3个具有完整开放阅读框的PpBBMs基因,其编码的蛋白均含有2个AP2结构域,其中,PpBBM1和PpBBM2具有相同的motif类型和数量。3个PpBBMs蛋白都是酸性蛋白,定位于细胞核,不存在跨膜结构和信号肽。系统进化分析表明,30个物种的BBM/BBML蛋白成员共聚为5类。其中,PpBBM1、PpBBM2聚在第2分支,分别与BdBBM和SbBBML亲缘关系最近;PpBBML位于第5分支,与ZmBBML亲缘关系最近。PpBBM1、PpBBM2和PpBBML均具有BBM基因所特有的bbm-1基序以及AP2亚家族特定的euANT2-4基序,但PpBBML蛋白的所有基序均有个别氨基酸残基存在变异。【结论】与PpBBML基因相比,PpBBM1和PpBBM2更有可能具有诱导孤雌生殖的功能。【Objective】Using bioinformatics methods,this paper aims to identify the embryogenic marker gene PpBBM of kentucky bluegrass and predict its parthenogenetic function through conservative motif alignment and evo⁃lutionary tree analysis.【Method】Based on the information from the Transcriptome database of kentucky bluegrass,the PpBBM gene was searched.The physical and chemical properties of PpBBMs protein were analyzed,and the phy⁃logenetic relationship and conservative motif differences of PpBBMs proteins were compared through bioinformatics software.【Result】A total of three PpBBMs genes with complete open reading frame were obtained,and the proteins encoded by them all contained two AP2 domains,among which PpBBM1 and PpBBM2 have the same motif type and number.The three PpBBMs proteins are acidic proteins located in the nucleus without transmembrane structure and signal peptide.Phylogenetic analysis showed that the menbers of BBM/BBML prdens of 30 species were polym⁃erized into five groups.Among them,PpBBM1 and PpBBM2 are clustered in the second branch and have the closest genetic relationships with BdBBM and SbBBML,respectively;PpBBML is located in the fifth branch and has the closest genetic relationship with ZmBBML.PpBBM1,PpBBM2,and PpBBML all have the bbm⁃1 motif unique to the BBM gene and the euANT2⁃4 motif specific to the AP2 subfamily.However,all motifs of the PpBBML protein exhibited variations in individual amino acid.【Conclusion】PpBBM1 and PpBBM2 genes are more likely to have the ability to induce parthenogenesis compared with PpBBML.

关 键 词：草地早熟禾 BBM基因 系统进化分析 保守基序 

分 类 号：S688.4[农业科学—观赏园艺]