牛乳铁蛋白对幽门螺杆菌根除作用的实验研究  

The functional effect of bovine lactoferrin on the eradication of Helicobacter pylori

作　　者：葛超毅 董锦沛 胡伏莲[1] 张继新[2] 李江[1] GE Chaoyi;DONG Jinpei;HU Fulian;ZHANG Jixin;LI Jiang(Department of Gastroenterology,Peking University First Hospital,Beijing 100034,China;Department of Pathology,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院消化内科,北京100034 [2]北京大学第一医院病理科,北京100034

出　　处：《胃肠病学和肝病学杂志》2024年第5期495-501,共7页Chinese Journal of Gastroenterology and Hepatology

基　　金：中国健康促进基金会(2019-TYSP-0121,2019-TYSP-0122)。

摘　　要：目的从体外和动物实验评价牛乳铁蛋白(bovine lactoferrin,bLF)对H.pylori的作用。方法将bLF分别与H.pylori标准菌株及临床耐药菌株共培养,记录bLF对H.pylori的最低抑菌浓度(minimum inhibitory concentration,MIC)值。构建H.pylori感染小鼠模型,评价加用bLF治疗后小鼠H.pylori根除情况及胃黏膜损伤程度、炎症因子及细胞间紧密连接蛋白变化情况。结果在bLF为1.5 g/L的浓度下能够抑制H.pylori标准菌株的生长,同时bLF在该浓度下能与抗生素协同抑制H.pylori耐药菌株生长;bLF能够减轻H.pylori感染小鼠胃黏膜炎症损伤程度,能够降低炎症因子TNF-α的水平,减少紧密连接蛋白Occludin和ZO-1的破坏。结论bLF具有一定的抗H.pylori作用,改善H.pylori造成的胃黏膜炎症程度,对胃黏膜细胞间紧密连接具有保护性作用。Objective To evaluate the effect of bovine lactoferrin(bLF)on H.pylori from in vitro and animal experiments.Methods bLF was co-cultured with H.pylori standard strains and drug-resistant strains,respectively,and the minimum inhibitory concentration(MIC)of bLF against H.pylori was recorded.A H.pylori infection mouse model was constructed to evaluate the H.pylori eradication and gastric mucosal damage,inflammatory factors and tight conjunctions in mice after bLF treatment.Results bLF could inhibit the growth of H.pylori standard strains at a concentration of 1.5 g/L.At the same concentration,bLF could cooperate with antibiotics to inhibit the growth of H.pylori resistant strains at this concentration;bLF could reduce the gastric mucosa of H.pylori infected mice.bLF could reduce the level of inflammatory factor TNF-α,prevent damage to tight junction proteins Occludin and ZO-1,and maintain mucosal integrity.Conclusion bLF has certain validity with anti-H.pylori effect.bLF can improve gastric mucosal inflammation caused by H.pylori and has a protective effect on the tight junction proteins between gastric mucosal cells.

关 键 词：牛乳铁蛋白 幽门螺杆菌 胃炎 根除治疗 

分 类 号：R378[医药卫生—病原生物学]