槐花外泌体减轻低氧诱导的胃和小肠黏膜急性氧化应激损伤研究  

Sophora japonica L.flower exosomes attenuate hypoxia-induced acute oxidative stress injury to the stomach and small intestine

作　　者：张恒 王德智 廖星晨 张明杰 王梦琪 许俊锋 王昕 金鹏 盛剑秋 ZHANG Heng;WANG Dezhi;LIAO Xingchen;ZHANG Mingjie;WANG Mengqi;XU Junfeng;WANG Xin;JIN Peng;SHENG Jianqiu(Medical School of Chinese PLA,Beijing 100853;Department of Gastroenterology,the Seventh Medical Center of Chinese PLA General Hospital;Senior Department of Gastroenterology,the First Medical Center of Chinese PLA General Hospital,China)

机构地区：[1]解放军医学院,北京100853 [2]中国人民解放军总医院第七医学中心消化内科 [3]中国人民解放军总医院第一医学中心消化内科医学部

出　　处：《胃肠病学和肝病学杂志》2024年第5期539-542,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：军事医学创新工程重点专项(18CXZ027)。

摘　　要：目的分析槐花外泌体在低氧诱导的胃和小肠黏膜急性氧化应激损伤中的作用。方法通过超速离心法提取槐花外泌体并完成电镜和粒径鉴定,采用低压氧环境控制系统构建小鼠低氧急性胃肠损伤模型,在低氧第1天给予槐花外泌体灌胃,第3天安乐死小鼠,收集消化道组织进行HE染色,收集胃和小肠黏膜进行ROS检测。期间记录小鼠的进食量、体质量、粪便性状和便血情况。结果口服槐花外泌体可改善低氧导致的小鼠血便、稀便和体质量下降,减轻低氧引起的胃和小肠黏膜损伤,降低ROS水平。结论口服槐花外泌体可有效缓解低氧引起的胃和小肠黏膜急性氧化应激损伤。Objective To analyze the role of Sophora japonica L.flower exosomes in hypoxia-induced acute oxidative stress injury to the stomach and small intestine.Methods Sophora japonica L.flower exosomes were extracted using ultracentrifugation followed by characterization through transmission electron microscopy and particle size measurement.Hypoxia-induced acute gastric and intestinal injury was triggered using placing mice in a ventilated hypoxic chamber system for 3 days.PBS or Sophora japonica L.flower exosomes were orally administered on day 1 under hypoxia.Mice were euthanized on day 3.Food consumption,body weight,stool score and stool blood score were recorded daily.The gastrointestinal tract was isolated for HE staining.The gastric and small intestinal mucosa was collected for ROS detection.Results Sophora japonica L.flower exosomes treatment ameliorated acute gastric and small intestinal mucosal injury and downregulated ROS induced by hypoxia.Conclusion Oral administration of Sophora japonica L.flower exosomes effectively attenuated hypoxia-induced acute oxidative stress injury to the stomach and small intestine.

关 键 词：槐花 外泌体 低氧 氧化应激 胃 小肠 

分 类 号：R57[医药卫生—消化系统]