火山岩载体对菖蒲脱氮作用的研究  

Effect of volcanic rock carrier on the removal of nitrogen by Acorus calamus L

作　　者：刘文婧 张秋奕 谭映宇 王洋 成水平[4] 王慧荣 王睿 江金兰 LIU Wenjing;ZHANG Qiuyi;TAN Yingyu;WANG Yang;CHENG Shuiping;WANG Huirong;WANG Rui;JIANG Jinlan(Eco-Environment Science Research&Design Institute of Zhejiang Province,Hangzhou Zhejiang 310007;Key Laboratory of Environmental Pollution Control Technology of Zhejiang Province,Hangzhou Zhejiang 310007;National Environmental Protection and Water Pollution Control Engineering Technology(Zhejiang)Center,Hangzhou Zhejiang 310007;College of Environmental Science and Engineering,Tongji University,Shanghai 200092;ZJMI Environmental Energy Co.,Ltd.,Hangzhou Zhejiang 310003;College of Geography and Environmental Sciences,Zhejiang Normal University,Jinhua Zhejiang 321004;College of Green and Intelligent Pharmaceutical Industry,Zhejiang Guangsha Vocational and Technical University of Construction,Jinhua Zhejiang 322103)

机构地区：[1]浙江省生态环境科学设计研究院,浙江杭州310007 [2]浙江省环境污染控制技术研究重点实验室,浙江杭州310007 [3]国家环境保护水污染控制工程技术(浙江)中心,浙江杭州310007 [4]同济大学环境科学与工程学院,上海200092 [5]浙江物产环保能源股份有限公司,浙江杭州310003 [6]浙江师范大学地理与环境科学学院,浙江金华321004 [7]浙江广厦建设职业技术大学绿色智能制药学院,浙江金华322103

出　　处：《环境污染与防治》2024年第5期659-665,670,共8页Environmental Pollution & Control

基　　金：浙江省“领雁”研发攻关计划项目(No.2023C03131);浙江省博士后择优资助项目(No.ZJ2022065);浙江省生态环境科研和成果推广项目(No.2021HT0060);2024年浙江省生态环境科学设计研究院院所扶持专项“近海河流氮素迁移转化规律及污染控制关键技术研发与示范”。

摘　　要：当前水生植物-微生物联合修复技术中,针对微生物载体作用的研究尚少见报道。因此,以供试挺水植物菖蒲(Acorus calamus L.)为研究对象,不同火山岩(黑色、灰色、红色)为微生物载体,考察菖蒲在火山岩载体下对水体总氮(TN)的去除效果,以及对菖蒲生物量、根系氮代谢酶活性和根际微生物多样性变化的影响。结果表明:种植菖蒲前,黑色火山岩载体人工湿地的TN去除率最高(40.8%);而种植菖蒲后,灰色火山岩人工湿地脱氮效果最佳,TN去除率可达70.2%,相较种植菖蒲前其TN、高锰酸盐指数、氨氮去除率分别提高了32.2、46.9、66.6百分点,根际微生物数量增加,出水单元菖蒲生长最旺盛,优势菌属为红环菌科未知属(unclassified_f_Rhodocyclaceae)。由此可见,含高SiO_(2)和低Fe_(2)O_(3)的灰色火山岩载体可增强菖蒲对污染水体的脱氮能力,为水体脱氮提供理论和技术支撑。The combination of aquatic plant-microorganism to purify water becomes an important means to control nitrogen pollution.However,the role of microbial carriers in it is still unclear.Therefore,the emergent macrophyte Acorus calamus L.was used.And three different groups of microorganism volcanic rock carriers(black,gray and red)were selected to discuss the effect of volcanic rock carrier on the TN removal efficiency of Acorus calamus L.,and the changes of Acorus calamus L.biomass,nitrogen metabolism enzyme activities from root and rhizospheric microbial community diversity were investigated.Result showed that:without planting,the black volcanic rock carrier had the highest TN removal efficiency(40.8%),while with planting,the gray volcanic rock carrier turned out to own the best denitrification effect,with a TN removal efficiency of 70.2%.Compared with no planting,the TN,permanganate index and ammonia nitrogen removal efficiency with gray volcanic rock carrier increased by 32.2,46.9,and 66.6 percentage points,respectively.Gray volcanic rock carrier also promoted the microbial activity in rhizosphere,and Acorus calamus L.growed most vigorously with dominant genus of unclassified_f_Rhodocyclaceae in outlet.Above all,the gray volcanic rock carrier with high SiO_(2)and low Fe_(2) O_(3)contents significantly enhanced the denitrification ability of Acorus calamus L.,which could provide the theoretical and technical support for nitrogen removal in water.

关 键 词：火山岩载体 总氮去除率 根际微生物 植物氮代谢相关酶 

分 类 号：X173[环境科学与工程—环境科学] X52