产微生物絮凝剂菌株的分离及絮凝特性研究  

Isolation of microbial flocculants producing strains and study on flocculation properties

作　　者：覃思绮 冯惠芳 刘一凤 艾国正 姜明国 刘国芳 QIN Siqi;FENG Huifang;LIU Yifeng;AI Guozheng;JIANG Mingguo;LIU Guofang(Guangxi Key Laboratory for Polysaccharide Materials and Modifications,School of Marine Sciences and Biotechnology,Guangxi Minzu University,Nanning Guangxi 530008)

机构地区：[1]广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西南宁530008

出　　处：《环境污染与防治》2024年第5期684-688,共5页Environmental Pollution & Control

基　　金：广西自然科学基金资助项目(No.2020GXNSFBA297140);广西壮族自治区区级大学生创新创业训练计划项目(No.S202210608161);广西民族大学研究生教育创新计划项目(No.gxun-chxs2022091)。

摘　　要：从某环保公司的污水处理设备好氧活性污泥和广西民族大学思源湖周边土壤中共分离出64个株菌,经过初筛和复筛得到14个絮凝率大于50%的株菌,最后对其中3个絮凝率最高的菌株进行了鉴定,分别属于蜡状芽孢杆菌(Bacillus cereus)、阿氏芽孢杆菌(Priestia aryabhattai)、拉尔宽假单胞菌(Pseudomonas lalkuanensis)。每50 mL 2.5 g/L高岭土悬液只需投加1.0 mL发酵液,蜡状芽孢杆菌在发酵时间为36 h、发酵pH为7.0时,絮凝率最高可达87.7%;阿氏芽孢杆菌在发酵时间为48 h、发酵pH为7.0时,絮凝率最高可达91.1%;拉尔宽假单胞菌在发酵时间为84 h、发酵pH为8.0时,絮凝率最高可达91.0%。3个产微生物絮凝剂菌株发酵条件比较温和,所需发酵液投加量少,表现出较高的絮凝率。因此,3个产微生物絮凝剂菌株具有实现工业化生产的潜在价值。64 strains were isolated from aerobic activated sludge of a sewage treatment equipment from an environmental protection company and soils around Siyuan Lake of Guangxi Minzu University.14 strains was screened after preliminary and secondary screenings.3 strains with the highest flocculation rate were identified as Bacillus cereus,Priestia aryabhattai and Pseudomonas lalkuanensis finally.Fermentation broth dosage of all the 3 strains only needed 1.0 mL per 50 mL 2.5 g/L kaoline turbid liquid.Bacillus cereus reached the highest flocculation rate of 87.7%,when fermentation time was 36 h and fermentation pH was 7.0.Priestia aryabhattai reached the highest flocculation rate of 91.1%,when fermentation time was 48 h and fermentation pH was 7.0.Pseudomonas lalkuanensis reached the highest flocculation rate of 91.0%,when fermentation time was 84 h and fermentation pH was 8.0.The results showed that the 3 strains had good flocculation performance when they were cultured at mild conditions and little fermentation broth dosage.Therefore,3 strains all had the potential to realize industrial production for microbial flocculants production.

关 键 词：菌株分离 菌株鉴定 微生物絮凝剂 絮凝特性 

分 类 号：X172[环境科学与工程—环境科学]