E3 ubiquitin ligase PbrATL18 is a positive factor in pear resistance to drought and Colletotrichum fructicola infection  

作　　者：Likun Lin Qiming Chen Kaili Yuan Caihua Xing Qinghai Qiao Xiaosan Huang Shaoling Zhang 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu,210095,China

出　　处：《Horticultural Plant Journal》2024年第3期698-712,共15页园艺学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2022YFD1200503);Jiangsu Agriculture Science and Technology Innovation Fund[Grant No.CX(22)3046];the National Science Foundation of China(Grant No.32072538);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Earmarked Fund for China Agriculture Research System(Grant No.CARS-28).

摘　　要：The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and development.Although the ATL family has been identified in other species,such as Arabidopsis,Oryza sativa,and grapevine,few reports on pear ATL gene families have been reported.In this study,92 PbrATL genes were identified and analyzed from the Pyrus breschneideri genome.Motif analysis and phylogenetic tree generation divided them into nine subgroups,and chromosome localization analysis showed that the 92 PbrATL genes were distributed in 16 of 17 pear chromosomes.Transcriptome data and quantitative real-time polymerase chain reaction(qRT-PCR)experiments demonstrated that PbrATL18,PbrATL41,and PbrATL88 were involved in both pear drought resistance and Colletotrichum fructicola infection.In addition,Arabidopsis thaliana overexpressing PbrATL18 showed greater resistance to drought stress than the wild type(WT),and PbrATL18-silenced pear seedlings showed greater sensitivity to drought and C.fructicola infection than the controls.PbrATL18 regulated plant resistance by regulating chitinase(CHI),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)activities.This study provided a reference for further exploring the functions of the PbrATL gene in drought resistance and C.fructicola infection.

关 键 词：PEAR Colletotrichum fructicola Arabidopsis Toxicos en Levadura(ATL) Drought stress 

分 类 号：S436.612.1[农业科学—农业昆虫与害虫防治]