JA-mediated MYC2/LOX/AOS feedback loop regulates osmotic stress response in tea plant  

作　　者：Junyan Zhu Hongrong Chen Lu Liu Xiaobo Xia Xiaomei Yan Xiaozeng Mi Shengrui Liu Chaoling Wei 

机构地区：[1]State Key Laboratory of Tea Plant Biology and Utilization/Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei,Anhui 230036,China

出　　处：《Horticultural Plant Journal》2024年第3期931-946,共16页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.32202542 and U20A2045);the Project of Major Science and Technology in Anhui Province(Grant No.202003a06020021);the Project of Science and Technology of Yunnan Province(Grant No.202102AE090038);Anhui Provincial Natural Science Foundation(Grant No.2108085QC121);the Natural Science Projects for Colleges and Universities in the Anhui Province(Grant No.KJ2021A0145)。

摘　　要：Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.

关 键 词：Camellia sinensis Jasmonic acid(JA) MYC2 transcription factor Lipoxygenase(LOX) Osmotic stress Peroxidase(POD) Reactive oxygen species(ROS) 

分 类 号：S571.1[农业科学—茶叶生产加工]