番茄花叶病毒种传机制研究及干热处理效果评价  

Study on the Seed Transmission Mechanism of Tomato Mosaic Virus and Evaluation of the Dry Heat Treatment Effect

作　　者：邱发慧 宋丽敏 刘蕾庆 林国祚 李彦辉 王忠全 夏世龙 QIU Fahui;SONG Limin;LIU Leiqing;LIN Guozuo;LI Yanhui;WANG Zhongquan;XIA Shilong(College of Plant Health&Medicine of Qingdao Agricultural University,Qingdao 266109,China;Qingdao Youbaili Agricultural Technology Co.,Ltd,Qingdao 266611,China)

机构地区：[1]青岛农业大学植物医学学院,山东青岛266109 [2]青岛优百粒农业科技有限公司,山东青岛266611

出　　处：《中国果菜》2024年第5期68-72,共5页China Fruit & Vegetable

基　　金：山东省科技型中小企业创新能力提升工程(2021TSGC1237);青岛市科技惠民示范引导专项(22-3-7-xdny-1-nsh)。

摘　　要：番茄花叶病毒(tomato mosaic virus,ToMV)是世界性分布的病毒,寄主范围广泛,番茄是其主要寄主,严重影响番茄生产。本研究以带毒的番茄种子为材料,通过逆转录聚合酶链反应(RT-PCR)检测病毒在番茄种子内的定位,采用干热方法处理种子后检测番茄种子的萌发率及ToMV的钝化率,旨在筛选出一个既可以有效钝化病毒,又不影响番茄种子萌发的处理条件,从源头上控制该病害的发生和流行。结果发现,ToMV定位于番茄种子的种皮,胚乳及胚中没有检测到该病毒的存在。对番茄种子进行干热处理,与未处理的番茄种子相比,75℃处理48 h和70℃处理96 h均会显著影响番茄种子的萌发,80℃处理24 h和65℃处理120 h对番茄种子的发芽率均没有显著影响。4个温度的恒温处理均可以有效降低种子携带ToMV的数量,但通过荧光定量PCR仍均能检测到ToMV。80℃处理24 h对ToMV的清除效果最好,且不影响番茄种子的发芽率。Tomato mosaic virus(ToMV)is a globally distributed virus that affects a wide range of hosts,with tomato being its primary host,significantly impacting tomato production.This study aimed to detect the localization of the virus in tomato seeds using RT-PCR.Additionally,it sought to assess the germination rate and ToMV inactivation rate following dry heat treatment,with the goal of identifying treatment conditions that effectively inactivate the virus while preserving the germination capacity of tomato seeds.ToMV was located in the seed coat of tomato seeds,with no detection in the endosperm or embryo.Compared with untreated tomato seeds,dry heat treatment at 75℃for 48 h and 70℃for 96 h significantly affected the germination of tomato seeds,while treatment at 80℃for 24 h and treatment at 65℃for 120 h did not significantly affect the germination rate.Constant temperature treatment at four different temperature effectively reduced the ToMV load in seeds,although the virus remained detectable by fluorescence quantitative PCR.Treatment at 80℃for 24 h exhibited the most effective removal of ToMV without compromising the germination rate of tomato seeds.

关 键 词：番茄花叶病毒 种传 干热处理 发芽率 

分 类 号：S339.31[农业科学—作物遗传育种]