聚苯乙烯纳米塑料(PS-NPs)对大豆生理指标及基因表达的影响  

Effects of polystyrene nanoplastics(PS-NPs)on physiological indexes and gene expression of soybean

作　　者：胡艳丽 王惠中 焦妍 李冬梅[3] 张子呈 姜妍[1,4] 佟晓红 王磊 王绍东[1] 王遂[1] HU Yan-li;WANG Hui-zhong;JIAO Yan;LI Dong-mei;ZHANG Zi-cheng;JIANG Yan;TONG Xiao-hong;WANG Lei;WANG Shao-dong;WANG Sui(Key Laboratory of Soybean Biology in Chinese Ministry of Education,Northeast Agricultural University,Harbin 150030,China;Jiamusi Agricultural and Rural Bureau,Jiamusi 154000,China;Crop Resources Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150030,China;National Research Center of Soybean Engineering and Technology,Harbin 150028,China;School of Resources and Environment,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]佳木斯农业农村局,黑龙江佳木斯154000 [3]黑龙江省农业科学院作物资源研究所,黑龙江哈尔滨150030 [4]东北农业大学国家大豆工程技术研究中心,黑龙江哈尔滨150028 [5]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《中国油料作物学报》2024年第2期284-293,共10页Chinese Journal of Oil Crop Sciences

基　　金：黑龙江省重点研发计划项目(2022ZX02B05);国家科技部“十四·五”子课题:优质大豆新种质创制与应用(2021YFD1201103-01-04);东北农业大学青年才俊项目(19QC01);大豆生物学教育部重点实验室开放课题(SBKF01)。

摘　　要：纳米塑料是一种新兴污染物,其对农作物的影响越来越受到关注。为探究大豆对聚苯乙烯纳米塑料(polystyrene nanoplastics,PS-NPs)在生理和基因表达水平上的响应,本试验采用水培方式,测定50 mg/L 100 nm的PS-NPs暴露处理10 d后大豆幼苗的表型及生理指标,并对大豆幼苗根系进行转录组测序分析。结果表明,PS-NPs暴露处理后,大豆幼苗生长受抑制,根系形态发生显著变化,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均一定程度增加,丙二醛(MDA)含量显著增加,可溶性糖含量减少。转录组分析表明,PS-NPs暴露处理共产生2112个差异基因,GO和KEGG分析显示其主要富集到与氧化还原反应、对乙烯和脱落酸的响应、氨基酸代谢和其他次生代谢物的合成相关的通路,注释到有250个转录因子发生差异表达,主要为ERF、WRKY、NAC、bHLH和MYB转录因子家族。本试验为进一步深入探究纳米塑料对大豆等农作物产生的影响及其分子机制奠定基础。As a novel environmental pollutant,the impacts of nanoplastics on crops have attracted more and more attention.To explore the response of soybean to polystyrene nanoplastics(PS-NPs)at physiological and gene expression levels,this experiment used hydroponics to determine the phenotype and physiological indexes of soybean seedlings after exposure to 50 mg/L 100 nm PS-NPs for 10 days,and carried out transcriptome sequencing analysis of soybean seedling root system.The results showed that the growth of the soybean seedlings was inhibited,the root morphology changed significantly,the activities of superoxide dismutase(SOD)and catalase(CAT)increased to a certain extent,the content of malondialdehyde(MDA)increased significantly,and the content of soluble sugar decreased after PS-NPs exposure.Transcriptome analysis showed that a total of 2112 differentially expressed genes were generated by PS-NPs exposure treatment,GO and KEGG analysis showed that they were mainly enriched in pathways related to redox reaction,reaction to ethylene and abscisic acid,amino acid metabolism and synthesis of other secondary metabolites.It was noted that 250 transcription factors were differentially expressed,mainly including the ERF,WRKY,NAC,bHLH and MYB transcription factor families.This experiment lays a foundation for further exploring the impact of nanoplastics on crops such as soybeans and its molecular mechanism.

关 键 词：大豆 纳米塑料 生理指标 基因表达 

分 类 号：S565.1[农业科学—作物学]