白介素1β及白介素6基因单核苷酸多态性与食管癌放疗后肺部感染的关联  

Association between gene single nucleotide polymorphisms of interleukin 1βand interleukin 6 and lung infection after radiotherapy for esophageal cancer

作　　者：杨静[1] 陈璐[1] 刘燕子 祝金冉 王胜根[1] YANG Jing;CHEN Lu;LIU Yanzi;ZHU Jinran;WANG Shenggen(Department of Oncology,The Third Affiliated Hospital of Xinxiang Medical College,Xinxiang 453000,China;Department of Clinical Laboratory,The Third Affiliated Hospital of Xinxiang Medical College,Xinxiang 453000,China)

机构地区：[1]新乡医学院第三附属医院肿瘤内科,新乡453000 [2]新乡医学院第三附属医院检验科,新乡453000

出　　处：《西南医科大学学报》2024年第3期236-240,250,共6页Journal of Southwest Medical University

基　　金：河南省医学科技攻关计划项目(LHGJ20190488)。

摘　　要：目的分析人白介素1β(interleukin 1β,IL-1β)、白介素6(interleukin 6,IL-6)基因单核苷酸多态性与食管癌放疗后肺部感染的关联,并探讨其生理病理的调控作用。方法选取新乡医学院第三附属医院2019年1月~2022年7月收治的115例食管癌患者为研究对象,根据放疗后是否出现感染分为感染组(n=28)和非感染组(n=87),检测IL-1β基因单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点-511C/T(rs16944)、-31C/T(rs1143627)和IL-6基因单核苷酸多态性位点IL-6-572C/G(rs1800796)、IL-6-174G/C(rs1800795)的多态性,采用Spearman相关检验分析IL-1β、IL-6基因单核苷酸多态性与食管癌放疗后肺部感染的关系,多因素Logistic回归分析影响食管癌放疗后肺部感染的相关因素。结果两组患者性别、年龄、BMI、临床分期等基线资料比较,差异无统计学意义(P>0.05);放疗时间、肿瘤长度、肿瘤位置比较差异有统计学意义(P<0.05);IL-1β基因的rs16944、rs1143627位点,IL-6基因的rs1800796、rs1800795位点的基因型分布均符合Hardy-Weinberg遗传平衡定律(P>0.05);两组IL-1β基因rs16944位点基因型频率,CC、CT、TT基因型频率,C、T等位基因频率,差异均无统计学意义(P>0.05);rs1143627位点CC、CT、TT基因型频率,C、T等位基因频率差异均有统计学意义(P<0.05);IL-6基因rs1800796位点CC、CG、GG基因型频率,C、G等位基因频率差异均有统计学意义(P<0.05),rs1800795位点CC、CG、GG基因型频率,C、G等位基因频率差异均无统计学意义(P>0.05);Spearman相关检验分析显示,IL-1βrs1143627(C/T)位点、IL-6 rs1800796(C/T)位点基因多态性与食管癌放疗后肺部感染具有显著相关性(P<0.05);多因素Logistic回归分析显示,放疗时间、肿瘤长度、肿瘤位置、IL-1βrs1143627位点、IL-6 rs1800796位点单核苷酸多态性是影响食管癌患者放疗后肺部感染的多因素(P<0.05)。结论IL-1β基因rs1143627(C/T)位点多态性及IL-6基因rs1800796(C/G)�Objective To analyze the association between human interleukin 1β(IL-1β)and interleukin 6(IL-6)gene single nucleotide polymorphisms and lung infection after radiotherapy for esophageal cancer,and to explore their physiological and pathologi⁃cal regulatory effects.Methods 115 patients with esophageal cancer treated in hospital between January 2019 and July 2022 were se⁃lected as the research subjects.According to whether lung infection occurred after radiotherapy,the patients were divided into lung in⁃fection group(n=28)and non-infection group(n=87).The single nucleotide polymorphisms(SNPs)at-511C/T(rs16944)and-31C/T(rs1143627)loci of IL-1βgene and at IL-6-572C/G(rs1800796)and IL-6-174G/C(rs1800795)loci of IL-6 gene were de⁃tected,and Spearman correlation test was used to analyze the relationship between gene polymorphisms of IL-1βand IL-6 and lung in⁃fection after radiotherapy for esophageal cancer.Multivariate Logistic regression analysis was performed to analyze the related factors of lung infection after radiotherapy for esophageal cancer.Results There were no statistically significant differences between the two groups of patients in terms of baseline data such as gender,age,BMI and clinical staging(P>0.05),and there were statistical differ⁃ences in radiotherapy time,tumor length and tumor location(P<0.05).The genotype distribution at rs16944 and rs1143627 loci of IL-1βgene and at rs1800796 and rs1800795 loci of IL-6 gene conformed to the Hardy-Weinberg genetic equilibrium law(P>0.05).The differences in the frequencies of CC,CT and TT genotypes and C and T alleles at rs16944 locus of IL-1βgene were not statistically sig⁃nificant between lung infection group and non-infection group(P>0.05),and the differences in frequencies of CC,CT and TT geno⁃types and C and T alleles at rs1143627 locus were statistically significant(P<0.05).There were statistical differences in the CC,CG and GG genotypes frequencies and C and G alleles frequencies at rs1800796 locus of IL-6 gene(P<0.05),and there were

关 键 词：白介素1Β 白介素6 基因多态性 食管癌 放疗 肺部感染 

分 类 号：R571[医药卫生—消化系统]