革兰氏阳性类芽孢杆菌(Paenibacillus sp.)的基因组和蛋白组研究  

Genome and proteome analysis of Paenibacillus sp.

作　　者：宋丽丽 杨小琴 张雪[2] 张更新[1] 计慕侃 张蕾 SONG Li-li;YANG Xiao-qin;ZHANG Xue;ZHANG Geng-xin;JI Mu-kan;ZHANG Lei(Key Laboratory of Tibetan Plateau Earth System,Environment and Resources,Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China;College of Ecology,Lanzhou University,Lanzhou 730000,China;Center for Pan-third Pole Enviroment,Lanzhou University,Lanzhou 730000,China;Academy of Science and Technology Development,Beijing University of Technology,Beijing 100101,China)

机构地区：[1]中国科学院青藏研究所青藏高原地球系统与资源环境重点实验室 [2]兰州大学生态学院 [3]兰州大学泛第三极环境中心 [4]北京工业大学科学技术发展院

出　　处：《应用化工》2024年第4期797-801,共5页Applied Chemical Industry

基　　金：第二次青藏高原综合科学考察研究专项(2019QZKK0503);国家自然科学基金项目(42030508,41871066,41471055)。

摘　　要：从西藏墨脱嘎隆拉山海拔3000m土壤中分离出一株革兰氏阳性类芽孢杆菌(Paenibacillus sp.),用Illumina Hiseq测序平台进行全基因组测序以及通过Prokka1.14.6软件进行基因组注释。结合antiSMASH 7.0和BAGEL 4分析Paenibacillus sp.基因组预测次级代谢产物生物合成基因簇。为了验证代谢产物路径,用不同破碎方法提取菌株全蛋白,通过蛋白质组质谱技术进行分析,结合不同蛋白质组分析软件,共鉴定了3383个蛋白质。通过同源比对,提取的蛋白质中可以检测到套索肽生物合成基因簇中依赖ATP半胱氨酸蛋白酶N端区域B1和ABC转运器。同时,用BAGEL4软件blastp功能从NCBI数据库中获取Paenibacillus sp.基因组中潜在完整的套索肽生物合成基因簇。It isolated a Gram positive Paenibacillus sp.strain from the soil at an altitude of 3 000 m in the Kalongla Mountains in Mutuo,Tibet.The entire genome of Paenibacillus sp.strain was sequenced by Illumina Hiseq and was annotated with Prokka software(v1.14.6).antiSMASH 7.0 and BAGEL4 were used to predict the secondary metabolites biosynthesis gene cluster in Paenibacillus sp.In order to validate the metabolic pathway,the total strain proteins were extracted by various extraction methods and performed nanoliter liquid chromatography combining with Mass Spectrometry.We could identify a total of 3 383 proteins by using software.Through homologous comparison,there were the N-terminal region of ATP dependent Cysteine protease B1 and ABC transporter in the lasso peptide biosynthesis gene cluster.It was complete by local blast comparison,and Paenibacillus sp.was likely to have the ability to synthesize lasso peptide.Through the above research,we analyed genomic and proteomic information of Paenibacillus sp.

关 键 词：类芽孢杆菌 基因组 蛋白质组 次级代谢产物 基因簇 

分 类 号：TQ458[化学工程—农药化工]