肝内胆管癌组织中CircACTN4 mRNA和THBS1mRNA的表达与临床病理特征及预后的关系研究  

Relationship between the Expression of CircACTN4 mRNA and THBS1mRNA in Intrahepatic Cholangiocarcinoma Tissue with Clinicopathological Features and Prognosis

作　　者：李顺乐 徐蒙 李蓉[2] 赵向菊 柴祎超 贾鸾 LI Shunle;XU Meng;LI Rong;ZHAO Xiangju;CHAI Yichao;JIA Luan(Department of General Surgery,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;Department of Anesthesiology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;Department of Oncology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;Department of Urology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院普通外科,西安710004 [2]西安交通大学第二附属医院麻醉科,西安710004 [3]西安交通大学第二附属医院肿瘤科,西安710004 [4]西安交通大学第二附属医院泌尿外科,西安710004

出　　处：《现代检验医学杂志》2024年第3期1-7,共7页Journal of Modern Laboratory Medicine

基　　金：陕西省2021年重点研发计划(项目编号:2021SF-229);陕西省2023年重点研发计划(项目编号:2023-YBSF-386);2023年陕西省科协青年人才托举计划(项目编号:20230301)。

摘　　要：目的研究肝内胆管癌(intrahepatic cholangiocarcinoma,ICC)中环状核糖核酸辅肌动蛋白α4(CircRNAsActininα4,Circ ACTN4)和血小板凝血酶蛋白1(platelet thrombin protein 1,THBS1)mRNA表达与临床病理特征和预后的关系,为临床诊治提供参考。方法回顾性分析2017年5月~2020年6月西安交通大学第二附属医院诊治的84例ICC患者;采用实时荧光定量PCR及免疫组织化学检测组织中CircACTN4 mRNA,THBS1 mRNA和蛋白表达。Pearson相关分析ICC癌组织中CircACTN4 mRNA与THBS1 mRNA的相关性。Kaplan-Meier法(Log-rank检验)比较不同CircACTN4mRNA,THBS1 mRNA表达ICC患者预后差异。COX回归分析ICC患者预后影响因素。受试者工作特征(ROC)曲线分析CircACTN4 mRNA,THBS1 mRNA对ICC患者死亡预后的评估价值。结果ICC癌组织中CircACTN4 mRNA(3.14±0.42)表达高于癌旁组织(0.76±0.25),差异具有统计学意义(t=44.094,P<0.001);ICC癌组织中THBS1 mRNA(2.82±0.36)和蛋白阳性率(92.86%)均高于癌旁组织(0.81±0.24,7.14%),差异具有统计学意义(t/χ2=42.068,123.429,均P<0.001)。ICC癌组织中CircACTN4 mRNA与THBS1 mRNA表达呈正相关(r=0.669,P<0.001)。TNM分期Ⅲ期、低分化程度及有淋巴结转移ICC癌组织中CircACTN4 mRNA,THBS1 mRNA表达高于TNM分期Ⅰ~Ⅱ期、高中分化程度及无淋巴结转移癌组织,差异具有统计学意义(χ2=7.949,9.164,12.207;23.270,18.625,19.828,均P<0.001)。CircACTN4 mRNA高表达组、THBS1 mRNA高表达组三年累积生存率分别低于CircACTN4 mRNA低表达组(25.00%vs 56.82%)和THBS1mRNA低表达组(19.51%vs 62.79%),差异具有统计学意义(Log rankχ2=13.601,24.310,均P<0.001)。CircACTN4mRNA(OR=1.839,95%CI:1.228~2.753)、THBS1 mRNA(OR=1.744,95%CI:1.245~2.443)、淋巴结转移(OR=1.925,95%CI:1.316~2.816)、TNM分期(OR=1.613,95%CI:1.223~2.126)及肿瘤分化程度(OR=1.510,95%CI:1.205~1.892)是影响ICC预后的独立因素(均P<0.01)。CircACTN4 mRNA,THBS1 mRNA联合检测对ICC患者死亡预后的评估曲线下面积为0.868,大�Objective To investigate the expression of circular RNA(Circ RNAS)Actininα4(ACTN4)and platelet thrombin protein 1(THBS1)in intrahepatic cholangiocarcinoma(ICC)and their relationship with clinicopathological characteristics and prognosis,and provide reference for clinical diagnosis and treatment.Method A retrospective analysis was conducted on 84 ICC patients diagnosed and treated in the Second Affiliated Hospital of Xian Jiaotong University from May 2017 to June 2020.The expressions of CircACTN4 mRNA,THBS1 mRNA and protein were detected by real-time fluorescent quantitative PCR and immunohistochemistry.Pearson correlation analysis was used to analyze the correlation between CircACTN4 mRNA and THBS1 mRNA in ICC cancer tissue.The prognostic differences of ICC patients with different CircACTN4 mRNA and THBS1 mRNA expressions were compared by the Kaplan-Meier method(log rank test).COX regression analysis was performed to identify prognostic factors in ICC patients.The prognostic value of CircACTN4 mRNA and THBS1 mRNA in evaluating the risk of death in ICC patients was assessed by receiver operating characteristic(ROC)curve analysis.Results The expression of CircACTN4 mRNA in ICC tissues(3.14±0.42)was higher than that in adjacent tissues(0.76±0.25),with significant difference(t=44.094,P<0.001).The positive rates of THBS1 mRNA(2.82±0.36)and protein positive rate(92.86%)in ICC tissues were higher than those in adjacent tissues(0.81±0.24,7.14%),and the differences were statistically significant(t/χ2=42.068,123.429,all P<0.001).CircACTN4 mRNA was positively correlated with THBS1 mRNA in ICC cancer tissue(r=0.669,P<0.001).The expressions of CircACTN4 mRNA,THBS1 mRNA in ICC cancer tissues with TNM stage III,low differentiation,and lymph node metastasis were higher than those in TNM stage I~II,high differentiation,and non-lymph node metastasis cancer tissues,and the differences were statistically significant(χ2=7.949,9.164,12.207;23.270,18.625,19.828,all P<0.001).The 3-year cumulative survival rates of the high expres

关 键 词：肝内胆管癌 环状核糖核酸辅肌动蛋白α4 血小板凝血酶蛋白1 

分 类 号：R735.7[医药卫生—肿瘤] R730.43[医药卫生—临床医学]