呼吸道产黑素普雷沃菌对小鼠Lewis移植瘤生长的影响  

Effects of Respiratory Prevotella Melaninogenica on the Growth of Lewis Transplanted Tumor in Mice

作　　者：王银璐 王华启[1] WANG Yinlu;WANG Huaqi(Department of Pulmonary and Critical Care Medicine,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第一附属医院呼吸与危重症医学科,河南郑州450000 [2]郑州大学,河南郑州450000

出　　处：《河南医学研究》2024年第10期1736-1740,共5页Henan Medical Research

摘　　要：目的 探讨肺癌患者肺部定植的产黑素普雷沃菌(PM)对小鼠Lewis移植瘤生长的影响。方法 购买6周龄的C57BL/6J雌性小鼠40只,随机选取30只构建小鼠Lewis移植瘤模型,10只作为对照组,至14 d时将模型组小鼠随机分为PBS组、PM1组、PM2组,在瘤周注射PBS或PM菌液,每周2次,观察小鼠肿瘤细胞生长情况;收集小鼠的肺泡灌洗液,应用16S rRNA扩增子测序技术,分析样本中微生物组成及微生物多样性。结果 PM1、PM2组小鼠肿瘤体积、质量均大于PBS组,差异有统计学意义(P<0.05);PM组小鼠肺泡灌洗液中微生物较对照组、PBS组丰度增加,Alpha多样性增多,Bete多样性降低。结论 小鼠肿瘤局部PM丰度增加显著促进小鼠Lewis移植瘤的生长,并且影响小鼠下呼吸道微生物菌群平衡。Objective To investigate the effect of Prevotella melaninogenica(PM)colonized in lung cancer patients on the growth of Lewis transplanted tumor in mice.Methods A total of 406-week-old C57BL/6J female mice were purchased,30 were randomly selected to construct Lewis transplanted tumor model,and 10 were used as control group.At 14 days,the model group of mice were randomly divided into PBS group,PM1 group and PM2 group,and PBS or PM bacterial solution was injected into the tumor peri-tumor twice a week.The growth of mouse tumor cells was observed.Alveolar lavage fluid was collected from mice,and the microbial composition and microbial diversity were analyzed by 16S rRNA amplicon sequencing.Results The volume and weight of tumor in PM1 and PM2 groups were higher than those in PBS group,and the difference was statistically significant(P<0.05).Compared with the control group and PBS group,the abundance of microorganisms in pulmonary alveolar lavage fluid of mice in PM group was increased,the diversity of Alpha was increased,and the diversity of Bete was decreased.Conclusion The increase of local PM in mouse tumor significantly promoted the growth of Lewis transplanted tumor,and affected the microflora balance in the lower respiratory tract of mice.

关 键 词：非小细胞肺癌 微生物 产黑素普雷沃菌 移植瘤 呼吸道菌群 

分 类 号：R734.2[医药卫生—肿瘤]