酵母多糖制备大鼠全身炎症反应综合征疾病动物模型  

Animal Model of Systemic Inflammatory Response Syndrome in Rats Prepared with Yeast Polysaccharide

作　　者：吴智婷 齐诗仪 龚萌 黄莹 吴靖雅 林锦文 林栋[1] Wu Zhiting;Qi Shiyi;Gong Meng(Fujian University of Chinese Medicine,Fuzhou,Fujian,350122,China)

机构地区：[1]福建中医药大学,福建福州350122

出　　处：《黑龙江医学》2024年第9期1052-1055,共4页Heilongjiang Medical Journal

基　　金：福建省自然科学基金项目(2020J01742)。

摘　　要：目的:通过研究使用酵母多糖来建立稳定的SIRS大鼠模型,为下一步探究全身炎症反应综合征(SIRS)发病机制及后续干预做准备。方法:Wistar大鼠均采用腹腔注射酵母多糖-石蜡混悬液制备SIRS动物模型,将模型组大鼠按照不同剂量分为400mg/kg、500mg/kg、600mg/kg、700mg/kg4组,通过对比观察,评估各组大鼠注射后的生存率(96h),给药后24h大鼠的一般情况、体温、白细胞(WBC)计数、外周血检测指标、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平以及肝肺脏器的病理苏木精-伊红(HE)染色。结果:腹腔注射酵母多糖-石蜡混悬液剂量为600mg/kg和700mg/kg的大鼠符合SIRS动物模型标准,600mg/kg组大鼠48h时生存率为40%,具有较长的生存周期;而700mg/kg组大鼠短期内的死亡率在24h后即可达到50%。结论:腹腔注射酵母多糖-石蜡混悬液是能成功制备较为稳定的SIRS模型大鼠的方法,600mg/kg剂量组的大鼠可存活的时间较长,故可用于SIRS发病机制研究,亦为SIRS的后续干预实验做好准备。Objective:To prepare for the next step of exploring the pathogenesis of SIRS and subsequent intervention,the experiment was conducted to establish a stable rat model of systemic inflammatory response syndrome(SIRS)by investigating the use of yeast polysaccharide.Methods:Wistar rats were all injected intraperitoneally with yeast polysaccharide-paraffin suspension to prepare the SIRS animal model,and the model group rats were divided into 4 groups of 400 mg/kg,500 mg/kg,600 mg/kg and 700 mg/kg according to different doses.The general condition,temperature,WBC,of rats 24h after administration and test index:TNF-α,IL-6 and pathological HE staining of liver and lung organs were evaluated.Results:Rats injected intraperitoneally with yeast polysaccharide-paraffin suspension at the doses of 600 mg/kg and 700 mg/kg met the criteria of SIRS animal model,and the survival rate of rats in the 600 mg/kg group was 40%at 48 h,which had a long survival cycle,while the mortality rate of rats in the 700 mg/kg group could reach 50%after 24 h in the short term.Conclusion:Intraperitoneal injection of yeast polysaccharide-paraffin suspension is a successful method to prepare more stable SIRS model rats,and the rats in the 600 mg/kg dose group can survive for a longer period of time.So it can be used to study the pathogenesis of SIRS and also prepare for the subsequent intervention experiments of SIRS.

关 键 词：酵母多糖 全身炎症反应综合征 模型 动物 大鼠 

分 类 号：R-332[医药卫生]