免疫组织化学室内质控对照组织的新型保存设置方法  

A new method of positive control tissue in immunohistochemistry

作　　者：白思施 王璐 Bai Sishi;Wang lu(Department of Pathology,the Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu 322000,China)

机构地区：[1]浙江大学医学院附属第四医院病理科,义乌322000

出　　处：《实用医技杂志》2024年第4期283-286,F0003,共5页Journal of Practical Medical Techniques

摘　　要：目的研究一种新型免疫组织化学阳性对照组织保存和设置方法,将目前在用的免疫组织化学阳性对照设置方法化繁为简。方法以胸腔积液为例,将新鲜胸腔积液离心固定破除红细胞,制成细胞悬液,然后将细胞悬液进行脱水处理,保存在按比例混合的石蜡和液体石蜡制成的膏状物中,灌注在唇膏管中,旋出膏体,涂抹在黏附玻片上,膏体中保存的细胞即可被黏附于玻片上,进行后续的免疫组织化学染色。结果显微镜下观察涂抹的细胞分布均匀,细胞密度适宜,细胞形态完整,在苏木精-伊红(HE)染色中,细胞核与细胞质染色对比清晰,细胞核着苏木精的紫色,细胞质着伊红的淡粉红色,组织细胞,淋巴细胞,间皮细胞等清晰可辨。在免疫组织化学CD68染色中,组织细胞细胞质棕黄色着色。在免疫组织化学Ki-67染色中,增殖期的细胞细胞核棕黄色着色。免疫组织化学染色结果表达清晰,定位准确。结论免疫组织化学阳性组织对照棒涂抹的阳性对照细胞可以代替蜡块切成蜡片制备的阳性对照,免疫组织化学阳性组织对照棒可以应用于免疫组织化学制片过程中阳性对照设置这一步骤中。Objective To study a new method of tissue preservation and preparation in immunohisto-chemical positive control and make the method of preparation of immunohistochemical positive control easier and more efficient.Methods Take pleuroperitoneal fluid as a specimen,centrifuge pleuroperitoneal fluid and then to break red blood cells,collect sedimentary cells,then dehydrate the cells,mix the cells with paraffin wax and liq-uid paraffin and then pour them into lip balm tube,smear it on adhesive glass slide,and then the cells in the cream can be adhered to the glass slide and use immunohistochemical stains finally.Results Using microscopes to observe cells,the cells are evenly distributed,and the shape of cells are integral.In HE staining,the nucleus and cytoplasm staining contrast are clear,the nucleus were purple with hematoxylin,the cytoplasm was pale pink with eosin,and the histiocytes,lymphocytes and mesothelial cells are clearly identifiable.In immunohistochemical CD68 staining,the nucleus of histiocytes were stained brown.In immunohistochemical Ki-67 staining,the nu-clear of cells in proliferation phase were stained brown.Immunohistochemical staining results showed clear ex-pression and accurate localization.Conclusion The tube of immunohistochemical positive control tissue can re-place traditional preparation of positive control,the tube of immunohistochemical positive control tissue can be used in the process of positive control preparation.

关 键 词：免疫组织化学 质量控制 阳性对照 抗原性 

分 类 号：R361.2[医药卫生—病理学]