橘绿木霉GF-11发酵刺梨渣的响应面法优化  

Optimization of Solid-State Fermentation Process of Trichoderma citrinoviride GF-11 on Rosa roxburghii Tratt Pomace by Response Surface Methodology

作　　者：杨昌发 唐彩艳 孙冰 张锐 费正林 葛永怡 YANG Changfa;TANG Caiyan;SUN Bing;ZHANG Rui;FEI Zhenglin;GE Yongyi(College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室,贵阳550025

出　　处：《中国生物防治学报》2024年第2期448-457,共10页Chinese Journal of Biological Control

基　　金：贵州烟草公司毕节市公司项目(201652050024145);黔科合平台人才([2017]5788-29)。

摘　　要：木霉具有抑制病原菌、促进植物生长和改善土壤营养等作用。刺梨渣是刺梨榨汁后的废弃物,具有极高的利用价值。本文拟采用刺梨渣为原料,麸皮为辅料进行固体发酵,以橘绿木霉GF-11的分生孢子数量为筛选指标,通过Plackett-Burman试验设计及Box-Behnken设计-响应曲面法(responsesurface methodology,RSM)对影响发酵刺梨渣的条件进行优化。结果表明,培养基配方中对橘绿木霉GF-11产孢影响最显著的3个主要因素麸皮占比、接种量、发酵天数。优化后的发酵培养条件为麸皮占比61%、接种量10.48%、硫酸锌0.5%、含水率250%、发酵天数10d。在此优化条件下发酵获得橘绿木霉菌株GF-11的分生孢子产量为2.32×10^(10)CFU/g。该研究为刺梨渣发酵提供了合适的工艺条件,为减少刺梨渣污染环境及其有效利用提供了基础,也为生产高质量生物有机肥提供了新的途径。Trichoderma spp.exhibit the capability to inhibit pathogen,promote plant growth,and enrich soil nutrition.The pomace derived from chestnut rose(Rose roxburghii Tratt.),a byproduct of chestnut rose juice processing,holds significant potential for valuable reutilization.This study aims to employ chestnut rose pomace as the primary substrate and wheat bran as the supplementary material for solid fermentation.The conidial quantity of Trichoderma citrinoviride GF-11 during solid-state fermentation was used as the screening criterion,with optimization of the fermentation conditions through a Plackett-Burman experimental design and a Box-Behnken design-response surface method.The results indicated that the proportion of bran,inoculum quantity,and fermentation duration are the key factors in the culture medium formula,exerting a significant impact on the sporulation of Trichoderma citrinoviride GF-11.The optimized fermentation culture conditions include wheat bran(61%),inoculation amount(10.48%),zinc sulfate(0.5%),moisture content(250%),and fermentation time(10 d).Under these specified conditions,the fermentation of Trichoderma citrinoviride GF-11 yielded 2.32×10^(10) CFU/g of conidia.This study not only provides suitable process conditions for the fermentation of chestnut rose pomace,and a pathway to decrease environmental pollution,but also enhance the efficient utilization of the pomace.Furthermore,it introduces a new approach for producing high-quality bio-organic fertilizer.

关 键 词：橘绿木霉 固体发酵 响应面法 刺梨渣 

分 类 号：S476[农业科学—农业昆虫与害虫防治]