不同频率电针对前交叉韧带损伤家兔股四头肌萎缩程度的影响  

Effects of Different Frequencies of Electroacupuncture on the Degree of Atrophy of the Quadriceps Femoris in Rabbits with Anterior Cruciate Ligament Injury

作　　者：黄永原 苏虹 张鹏翼 韦业腾 徐照琳 伏春华[3] 陈慧姣 杨雪捷 李嘉英 王晨玺 HUANG Yongyuan;SU Hong;ZHANG Pengyi;WEI Yeteng;XU Zhaolin;FU Chunhua;CHEN Huijiao;YANG Xuejie;LI Jiaying;WANG Chenxi(College of Acupuncture and Moxibustion,Guangxi University of Chinese Medicine,Nanning 530000,China;Graduate School of Guangxi University of Chinese Medicine,Nanning 530000,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530000,China)

机构地区：[1]广西中医药大学针灸推拿学院,广西南宁530000 [2]广西中医药大学研究生院,广西南宁530000 [3]广西中医药大学第一附属医院,广西南宁530000

出　　处：《中国中医药信息杂志》2024年第6期88-94,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81960899)。

摘　　要：目的观察不同频率电针对前交叉韧带(ACL)损伤模型家兔股四头肌萎缩程度的影响,探究电针治疗ACL损伤的可能机制。方法24只新西兰兔随机分为空白组、模型组、低频电针组和高频电针组,每组6只。除空白组外,其余组建立ACL损伤模型。造模后第7日,低频电针组和高频电针组取术侧“血海”“梁丘”行电针刺激(连续波,频率2、100Hz,留针20min),其余各组仅抓取固定,连续21d。计算股四头肌质量比,HE染色观察股四头肌组织形态,ELISA检测股四头肌组织一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)含量,荧光探针法检测股四头肌组织活性氧(ROS)含量,Westernblot检测股四头肌组织蛋白激酶R样内质网激酶(PERK)、转录激活因子6(ATF6)、肌醇依赖酶1(IRE1)、肌肉特异性环脂蛋白1(MuRF1)和肌萎缩蛋白(MAFbx)蛋白表达。结果与空白组比较,模型组家兔股四头肌质量比明显减少(P<0.01),肌细胞排列不规则,伴肿大、萎缩,间质水肿明显,大面积炎性浸润,股四头肌组织NO、iNOS、ROS含量明显增加(P<0.01),PERK、ATF6、IRE1、MuRF1、MAFbx蛋白表达明显升高(P<0.01);与模型组比较,低频电针组和高频电针组股四头肌质量比明显增加(P<0.01),肌细胞排列较规律,细胞肿大、萎缩情况好转,间质水肿、炎症均减轻,股四头肌组织NO、iNOS、ROS含量明显减少(P<0.01),PERK、ATF6、IRE1、MuRF1、MAFbx蛋白表达明显降低(P<0.01),且低频电针组效果优于高频电针组(P<0.05)。结论不同频率电针干预可延缓ACL损伤家兔股四头肌萎缩程度,其机制可能与减少股四头肌氧化代谢产物NO、iNOS、ROS和内质网应激相关蛋白PERK、ATF6、IRE1表达,进而抑制肌萎缩因子MuRF1、MAFbx表达,延缓股四头肌蛋白降解有关,且低频电针效果优于高频电针。Objective To observe the effects of different frequencies of electroacupuncture on the degree of atrophy of quadriceps femoris in rabbits with anterior cruciate ligament(ACL)injury model;To explore the possible mechanisms of electroacupuncture in treating ACL injury.Methods Totally 24 New Zealand rabbits were randomly divided into blank group,model group,low-frequency electroacupuncture group and high-frequency electroacupuncture group,with 6 rabbits in each group.Except for the blank group,ACL injury models were established in the other groups.On the 7th day after modeling,the low-frequency electroacupuncture group and the high-frequency electroacupuncture group performed electroacupuncture treatment(continuous wave,frequency of 2 Hz and 100 Hz respectively,and left the needle in place for 20 min)at“Xuehai”and“Liangqiu”of the operation side,and the remaining groups were only grasped and immobilized,for 21 days.The mass ratio of the quadriceps femoris was calculated,the histopathological morphology of the quadriceps femoris tissue of the rabbits in each group was observed by HE staining,the expressions of NO,iNOS in quadriceps femoris tissue were detected by ELISA,the content of ROS in quadriceps femoris tissue was detected by fluorescent probe,the protein expressions of PERK,ATF6,IRE1,MuRF1,MAFbx in quadriceps femoris tissue were detected by Western blot.Results Compared with the blank group,the quadriceps femoris muscle mass ratio of the model group rabbits was significantly reduced(P<0.01),with irregular arrangement of muscle cells,accompanied by swelling and atrophy,significant interstitial edema,and extensive inflammatory infiltration,the contents of NO,iNOS and ROS in quadriceps femoris tissue significantly increased(P<0.01),while the expressions of PERK,ATF6,IRE1,MuRF1 and MAFbx proteins significantly increased(P<0.01).Compared with the model group,the quadriceps femoris muscle mass ratio in the low-frequency and high-frequency electroacupuncture groups significantly increased(P<0.01),with regular

关 键 词：前交叉韧带损伤 肌萎缩 电针 氧化应激 内质网应激 兔 

分 类 号：R245.3[医药卫生—针灸推拿学]