miR-29c-3p调控ERLIN2抑制肺腺癌发展的作用  

Role of miR-29c-3p in regulating ERLIN2 to suppress the development of lung adenocarcinoma

作　　者：卞婷婷[1] 姜岱山 张雅莉 王斯楚 刘益飞[1] BIAN Tingting;JIANG Daishan;ZHANG Yali;WANG Sichu;LIU Yifei(Department of Pathology,Affiliated Hospital of Nantong University,Jiangsu 226001;Department of Emergency Medicine,Affiliated Hospital of Nantong University,Jiangsu 226001)

机构地区：[1]南通大学附属医院病理科,江苏226001 [2]南通大学附属医院急诊医学科,江苏226001

出　　处：《交通医学》2024年第2期116-120,125,共6页Medical Journal of Communications

基　　金：南通市科技项目(MSZ19164,JCZ20001,JC12022016);新锐肿瘤支持科研专项(cphcf-2022-206)。

摘　　要：目的:探讨miR-29c-3p在肺腺癌(lung adenocarcinoma,LUAD)发生发展中的作用。方法:利用公共数据库分析miR-29c-3p和ERLIN2在肺腺癌中的表达及其与临床病理参数的相关性。采用qRT-PCR检测miR-29c-3p在LUAD细胞系和正常支气管上皮细胞系中的表达,CCK8、细胞克隆形成、Transwell和伤口愈合实验评估miR-29c-3p在细胞增殖、迁移和侵袭中的作用,利用生物信息学工具预测miR-29c-3p下游靶基因,并通过双荧光素酶报告基因分析验证靶向关系。结果:miR-29c-3p在肺腺癌组织和细胞中低表达。miR-29c-3p低表达患者的预后较差,miR-29c-3p是肺腺癌患者的独立预后因素,并与淋巴结转移状态和临床分期密切相关。细胞实验表明,抑制miR-29c-3p可促进A549细胞的增殖、迁移和侵袭。生物信息学分析表明,ERLIN2是miR-29c-3p的靶基因,二者表达呈负相关。通过双荧光素酶报告基因测定验证了上述靶向关系。在UALCAN数据库中,ERLIN2在肺腺癌组织中高表达,并与患者年龄、性别、吸烟、肿瘤分期、淋巴结转移和TP53突变状态密切相关,ERLIN2高表达患者总生存率低于低表达患者。结论:miR-29c-3p通过靶向ERLIN2抑制肺腺癌细胞的增殖、迁移和侵袭,是肺腺癌患者的独立预后因素。Objective:To explore the impact of miR-29c-3p in the development of lung adenocarcinoma.Methods:Public database was used to analyze the expression of miR-29c-3p and ERLIN2 in lung adenocarcinoma and its correlation with clinicopathological parameters.qRT-PCR was used to detect the expression of miR-29c-3p in LUAD cell line and normal bronchial epithelial cell line.The function of miR-29c-3p in cell proliferation,migration,and invasion was evaluated by CCK8,colony formation,Transwell assay and wound healing essay.Bioinformatics predicted the downstream target genes of miR-29c-3p,and verified the targeting relationship by Dual luciferase reporter assay.Results:miR-29c-3p was low expressed in lung adenocarcinoma tissues and cells.miR-29c-3p was an independent prognostic factor for lung adenocarcinoma and was closely related to lymph node metastasis status and clinical staging.Celluar experiments demonstrated that miR-29c-3p inhibitor promoted the proliferation,migration,and invasion of A549 cells.Bioinformatics analysis showed that ERLIN2 was a target gene for miR-29c-3p,and there was a negative correlation between their expression.The above targeting relationship was verified through dual luciferase reporter assay.In the UALCAN database,ERLIN2 is highly expressed in tumor tissue and is closely related to patient age,gender,smoking habits,tumor staging,lymph node metastasis status,and TP53 mutation status.Meanwhile,the higher the expression of ERLIN2,the shorter the overall survival time of patients.Conclusions:miR-29c-3p inhibits the proliferation,migration,and invasion of lung adenocarcinoma by targeting ERLIN2,it is an independent prognosis factor.

关 键 词：肺腺癌 miR-29c-3p ERLIN2 增殖 侵袭 迁移 

分 类 号：R734.2[医药卫生—肿瘤]