绵羊MTPN基因多态性与生长性状的关联分析  

Association between MTPN Gene Polymorphism and Growth Traits in Sheep

作　　者：郑菲 黄志 王婧尊 祁高展[1] 徐红伟[1,2] 高丹丹[1,2] 郭鹏辉[1,2] 曹忻 杨具田[1,2] 蔡勇[1,2,3] Zheng Fei;Huang Zhi;Wang Jingzun;Qi Gaozhan;Xu Hongwei;Gao Dandan;Guo Penghui;Cao Xin;Yang Jutian;Cai Yong(College of Life Science and Engineering,Northwest Minzu University,Lan Zhou,730030,Gan Su;Ecological Industry Development Research Institute of the Upper Yellow River,Northwest Minzu University,Lan Zhou,730030,Gan Su;Department of Experimental Teaching,Northwest Minzu University,Lan Zhou,730030,Gan Su)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学黄河上游生态产业发展研究院,甘肃兰州730030 [3]西北民族大学实验教学部,甘肃兰州730030

出　　处：《中国畜禽种业》2024年第4期39-47,共9页The Chinese Livestock and Poultry Breeding

基　　金：中央高校基本科研业务费专项(31920230166);中央高校基本科研业务费专项(31920220057);甘肃省自然科学基金项目(20JR10RA122);国家民委中青年英才培养计划项目(民人事字[2022] 366);甘肃省高校产业支撑计划项目(2023CYZC-08)。

摘　　要：为了研究肌侵蛋白(MTPN)基因多态性与绵羊生长性状相关性,挖掘与生长相关的分子遗传标记,该试验以1557只绵羊作为研究对象,包括兰州大尾羊536只,滩羊904只、藏绵羊117只,采用PCR-SSCP结合测序技术识别单核苷酸多态位点并进行基因分型,探究这些突变位点与不同生长性状之间的可能关联。结果表明:兰州大尾羊、滩羊、藏绵羊群体中均存在g.101185485G>A变异位点,该位点存在AA、 AG两种基因型。三个绵羊群体中g.101185485 G>A位点均属于低度多态(PIC<0.25),处于Hard-Weinberg平衡状态(P>0.05)。在兰州大尾羊、滩羊和藏绵羊中,AA基因型为优势基因型,AA基因型兰州大尾羊的体高和管围、滩羊的体高和胸围以及藏绵羊的体高均显著高于AG基因型(P<0.05)。可见,MTPN基因g.101185485 G>A变异位点可作为绵羊分子选育过程中的有效遗传标记位点,在选育工作中优先考虑。To investigate the correlation between myotrophin(MTPN)gene polymorphisms and growth characteristics in sheep,molecular genetic markers linked to growth were scrutinized.A total of 1557 sheep were enlisted for this inquiry,comprising 536 specimens of Lanzhou large-tailed sheep,904 Tan sheep,and 117 Tibetan sheep.PCR-SSCP in conjunction with sequencing was employed to discern single nucleotide polymorphisms and genotypes,to delve into the potential correlation between these mutational sites and various growth traits.The findings revealed the presence of a variant locus,g.101185485G>A,in Lanzhou large-tailed sheep,Tan sheep,and Tibetan sheep,with AA and AG genotypes observed at this locus.The g.101185485 G>A locus exhibited low polymorphism(PIC<0.25)and was in Hardy-Weinberg equilibrium(P>0.05)across all three sheep populations.Among Lanzhou large-tailed sheep,Tan sheep,and Tibetan sheep,the AA genotype prevailed,exhibiting significantly greater body height and chest circumference in Lanzhou large-tailed sheep,body height and chest circumference in Tan sheep,and body height in Tibetan sheep with the AA genotype compared to those with AG genotype(P<0.05).Thus,it is evident that the g.101185485 G>A variant of the MTPN gene can serve as a valuable genetic marker locus in the molecular breeding of sheep and is favored in breeding endeavors.

关 键 词：绵羊 MTPN基因多态性 生长性状 关联分析 

分 类 号：S826[农业科学—畜牧学]