黄芪皂苷Ⅱ对肾透明细胞癌细胞迁移和侵袭能力的抑制作用及其机制  

Inhibitory effects of astragalosideⅡon invasion and migration of clear cell renal cell carcinoma

作　　者：赵凯[1] 李勋华 王科[1] ZHAO Kai;LI Xunhua;WANG Ke(Department of Urology,Affiliated Hospital of Qingdao University,Qingdao 266003,China)

机构地区：[1]青岛大学附属医院泌尿外科,山东青岛266003

出　　处：《山东医药》2024年第14期6-9,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(31971191)。

摘　　要：目的 探讨黄芪皂苷Ⅱ(ASⅡ)对肾透明细胞癌细胞迁移和侵袭的抑制作用,并分析该作用与Wnt/β-catenin信号通路的关系。方法 取人肾透明细胞癌细胞786-O,随机分为对照组、ASⅡ组和LiCl干预组。对照组仅更换新鲜血清培养基,不进行其他处理;ASⅡ组加入100μmol/L ASⅡ溶液;LiCl干预组先加入10 mmol/L LiCl(Wnt/β-catenin信号通路激活剂)溶液,24 h后加入100μmol/L ASⅡ溶液。采用细胞划痕实验观察各组细胞迁移能力,Transwell实验观察细胞侵袭能力,Western blotting法检测细胞上皮间充质转化(EMT)相关蛋白E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)。结果 与对照组比较,ASⅡ组细胞迁移率降低;与ASⅡ组比较,LiCl干预组细胞迁移率升高(P均<0.01)。与对照组比较,ASⅡ组穿膜细胞数减少;与ASⅡ组比较,LiCl干预组穿膜细胞数增加(P均<0.01)。与对照组比较,ASⅡ组E-cadherin蛋白表达水平升高,N-cadherin、Vimentin及β-catenin蛋白表达水平降低;与ASⅡ组比较,LiCl干预组E-cadherin蛋白表达水平降低,N-cadherin、Vimentin及β-catenin蛋白表达水平升高(P均<0.01)。结论 ASⅡ能够抑制肾透明细胞癌细胞的迁移和侵袭能力,其机制可能是通过调控Wnt/β-catenin信号通路来抑制细胞EMT实现的。Objective To explore the inhibitory effects of astragaloside II(ASⅡ)on invasion and migration of clear cell renal cell carcinoma cells,and to analyze the relationships between the effects and Wnt/β-catenin signaling pathway.Methods Human clear cell renal cell carcinoma cells(786-O)were taken and were randomly divided into three groups:the control group,the ASⅡgroup,and the LiCl intervention group.In the control group,only fresh serum was used to re⁃place the culture medium throughout the experiment without any other treatments;in the ASⅡgroup,100μmol/L ASⅡsolution was added to the cells;in the LiCl intervention group,cells were treated with 10 mmol/L LiCl solution,and after 24 h,they were added with 100μmol/L ASⅡsolution.The migration ability of the cells in each group was measured by Scratch assay,the invasive ability of the cells was detected by Transwell assay,and epithelial-mesenchymal transition(EMT)-related proteins E-cadherin,N-cadherin,and N-cadherin were detected by Western blotting.Results Com⁃pared with the control group,the cell migration rate decreased in the ASⅡgroup;compared with the ASⅡgroup,the cell migration rate increased in the LiCl intervention group(both P<0.01).Compared with the control group,the number of transmembrane cells decreased in the ASⅡgroup;compared with the ASⅡgroup,the number of transmembrane cells in⁃creased in the LiCl intervention group(both P<0.01).Compared with the control group,the expression level of E-cad⁃herin protein increased in the ASⅡgroup,while the protein expression levels of N-cadherin,Vimentin andβ-catenin de⁃creased.Compared with the ASⅡgroup,the expression level of E-cadherin protein in the LiCl intervention group de⁃creased,while the protein expression levels of N-cadherin,Vimentin andβ-catenin increased(all P<0.01).Conclusions ASⅡcan significantly inhibit the invasion and migration of clear cell renal clear carcinoma.Its mechanism may be that AS Ⅱ can inhibit EMT via regulating Wnt/β-catenin signaling pathway.

关 键 词：黄芪皂苷Ⅱ 肾肿瘤 WNT/Β-CATENIN信号通路 细胞迁移 细胞侵袭 上皮间充质转化 

分 类 号：R737.11[医药卫生—肿瘤]