有氧运动通过Keap1Nrf2HO-1通路对睡眠障碍老年大鼠认知的改善机制  

Cognitive improvement mechanism of aerobic exercise via Keap1Nrf2HO-1 pathway in sleep-disordered aged rats

作　　者：陈晓勇[1,2] 李洁华[1] 赵丽花[2] 赵家龙 颜闪闪 林紫薇 高理升[3] CHEN Xiaoyong;LI Jiehua;ZHAO Lihua;ZHAO Jialong;YAN Shanshan;LIN Ziwei;GAO Lisheng(Department of Gerontology Cardiology,The First Affiliated Hospital of Anhui Medical University,Hefei 230032,Anhui,China;General Medicine Department,The People's Hospital Bozhou,Bozhou 236800,Anhui,China;Intelligent Machinery Research Institute,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031 Anhui,China)

机构地区：[1]安徽医科大学第一附属医院老年心血管内科,合肥230032 [2]亳州市人民医院全科医学科,亳州236800 [3]中国科学院合肥物理研究所智能机械研究所,合肥230031

出　　处：《医学研究与战创伤救治》2024年第2期125-129,共5页Journal of Medical Research & Combat Trauma Care

基　　金：国家重点研发计划项目(2020YFC2005603)。

摘　　要：目的探究有氧运动通过Keap1Nrf2HO-1通路对睡眠障碍老年大鼠认知的改善机制。方法选用Wistar大鼠,60只,雌雄各半,将大鼠均分为4组,其中一组为空白组,其余分为模型组、给药组(根据每只大鼠的体重按照0.5 mg/kg进行艾司唑仑片灌胃给药)和有氧运动组,每组15只。结果与空白组比较,模型组逃避潜伏期延长(P<0.01),且伴随着明显的寻台次数较少、穿越原平台次数降低(P<0.01),大鼠在原平台停留的时间占总游泳时间的百分比降低(P<0.05);与模型组比较,给药组和有氧运动组逃避潜伏期缩短(P<0.01),且伴随着明显的寻台次数增加、穿越原平台次数增加(P<0.01),在原平台停留的时间占总游泳时间的百分比升高(P<0.05)。与空白组比较,模型组MDA水平升高,SOD活性降低(P<0.01),Keap1水平升高(P<0.01),Nrf2和HO-1水平降低(P<0.01);与模型组比较,给药组和有氧运动组MDA水平降低,SOD活性升高(P<0.01),Keap1水平降低,Nrf2和HO-1水平升高(P<0.01)。结论有氧运动能够有效缓解失眠障碍大鼠的认知功能,其机制原理可能与有氧运动通过改善睡眠障碍大鼠机体内海马组织的Keap1/Nrf2/HO-1蛋白表达水平从而产生抗氧化应急作用有关。Objective To explore the cognitive improvement mechanism of aerobic exercise in elderly rats with sleep disorders through Keap1Nrf2H0-1 pathway.Methods 60 Wistar rats(30 male and 30 female rats)were divided into 4 groups,the blank group,model group,drug group(according to the qualiy of each rat's body shall be caried out in accordance with the O.5 mg/kg estazolam lavage administration)and aerobic exercise group.Each group consisted of 15 rats.Results Compared with the blank group,the escape latency of the model group was prolonged(P<0.01),accompanied by a lower number of platform searches,a lower number of traversals(P<0.01),and a lower percentage of the total swimming time spent on the original platform(P<0.05);while the escape latency of the drug-administered group and the aerobic group was shortened(P<0.01),accompanied by an increase in the number of platform searches,an increase in the number of traversals(P<0.01),and a higher percentage of the total swimming time spent on the original platform(P<0.05).The escape latency was shorter(P<0.01)in the drug administration group and the aerobic exercise group,and was accompanied by a significant increase in the number of plaform searches,an increase in the number of crossings of the original platform(P<0.01),and an increase in the percentage of the total swimming time spent on the original platform(P<0.05).Compared with the blank group,the model group showed higher MDA level,lower SOD activity(P<0.01),higher Keapl level(P<0.01),and lower Nrf2 and HO-1 levels(P<0.01),while the drug-administered and aerobic exercise groups showed lower MDA level,higher SOD activity(P<0.01),lower Keap1 level,and higher Nr2 and H0-1 levels(P<0.01).-llevels were elevated(P<0.01).Conclusion Aerobic exercise can improve the cognitive function of rats with insomnia disorder,which maybe related to the antioxidant emergency effect of aerobic exercise by upregulatingthe expression levels of Keap1/Nrf2/HO-1 protein in the hippocampus of rats with sleep disorder.

关 键 词：有氧运动 Keap1/Nrf2/HO-1蛋白 睡眠障碍 大鼠 改善机制 

分 类 号：R749[医药卫生—神经病学与精神病学]