大鼠严重腹腔感染后脾脏细胞周期的变化  

Change of cell cycle in rat spleen after severe abdominal infection

作　　者：李晋平 刘汉卿 杨全会 LI Jinping;LIU Hanqing;YANG Quanhui(Department of Critical Medical Sciences/National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021;Department of Critical Medical Sciences,Tangshan Huatuo Hospital,Tangshan 063500,China)

机构地区：[1]国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院、北京协和医学院肿瘤医院、重症医学科,北京100021 [2]河北省唐山华佗医院重症医学科,河北唐山063500

出　　处：《基础医学与临床》2024年第6期828-832,共5页Basic and Clinical Medicine

基　　金：分子肿瘤学国家重点实验室开放课题(SKL-KF-2019-2)。

摘　　要：目的探讨大鼠腹腔感染时动物的死亡率,脾脏细胞周期和细胞凋亡的变化。方法采用盲肠结扎穿孔术(CLP)制备大鼠腹腔感染的动物模型,计算术后0 h、6 h、12 h、24 h、48 h和72 h动物的死亡率,流式细胞仪检测脾脏实质细胞细胞周期及细胞凋亡的变化,Western_blot法检测不同时间点脾脏细胞周期素依赖性激酶的抑制因子p27蛋白的表达。结果在大鼠CLP诱导的全身炎症反应中,CLP术后0 h、6 h、12 h、24 h、48 h、72 h动物死亡率逐渐增加,最高达88.81%,CLP术后0 h、6 h、12 h、24 h动物腹腔感染逐渐加重,48 h及72 h后腹腔炎性局限并逐渐减轻。在CLP术后48 h内脾脏细胞出现周期G1阻滞、凋亡细胞百分数增加、p27表达增加;48 h后细胞周期G1期阻滞逐渐恢复,凋亡细胞逐渐减少,p27表达减少。在CLP术后48 h内脾脏细胞周期S和G2/M期细胞百分数减少,48 h后S和G2/M期细胞百分数逐渐增加。结论脾脏细胞周期阻滞和细胞凋亡可能参与了大鼠腹腔感染后动物死亡率变化。Objective To explore the mortality rate and changes in splenic cell cycle and apoptosis in rats with abdominal infection.Methods An animal model of sepsis was induced by cecal ligation and puncture(CLP)in rats.At serial time points 0 h,6 h,12 h,24 h,48 h,and 72 h after CLP,the animal death rate was calculated.The percentage of cell cycle G1,S,G2/M phase and apoptosis of isolated spleen cells were analyzed using flow cytometer.The expression of p27 of spleen tissue was determined by Western blot analysis.Results In the rat model of CLP-induced systemic inflammatory response,the mortality of animals gradually increased at 0 h,6 h,12 h,24 h,48 h and 72 h after CLP surgery,reaching a maximum of 88.81%.The abdominal infection in the animals gradually worsened at 0 h,6 h,12 h,and 24 h after CLP surgery,but localized and gradually alleviated after 48 h and 72 h.Within 48 h after CLP surgery,there was a blockage in the G1 phase of the spleen cell cycle,an increase in the percentage of apoptotic cells,and an elevation in p27 expression.After 48 h,the G1 phase blockage gradually recovered,the number of apoptotic cell decreased,and p27 expression declined.Additionally,within 48 h after CLP surgery,there was a decrease in the percentage of cells in the S and G2/M phases of the spleen cell cycle,but the percentage of cells in these phases gradually increased after 48 h.Conclusions Cell cycle arrest and apoptosis of the spleen cells are potentially contributed to the change of animal mortality after abdominal infection.

关 键 词：脾脏 严重感染 细胞周期 细胞凋亡 P27 

分 类 号：R363.1[医药卫生—病理学]