急性脑梗死患者外周血STAT6和PPARα动态变化与病情严重程度及预后的关系分析  

Dynamic changes of signal transducer and activator of transcription 6 and peroxisome proliferator-activated receptor alpha in peripheral blood of patients with acute cerebral infarction and their association with disease severity and prognosis

作　　者：李娜 郭锋[1] 刘文运[1] 马济滨[1] LI Na;GUO Feng;LIU Wenyun(Department of Neurology,Lyuliang People's Hospital,Lyuliang 033000,China)

机构地区：[1]吕梁市人民医院神经内科,山西吕梁033000

出　　处：《中风与神经疾病杂志》2024年第5期464-469,共6页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨急性脑梗死(ACI)患者外周血信号转导和转录激活因子6(STAT6)和过氧化物酶体增殖物激活受体α(PPARα)动态变化与病情严重程度及预后的关系。方法选取2021年1月―2022年6月在吕梁市人民医院住院治疗的86例ACI患者为研究对象为ACI组,然后通过美国国立卫生研究院卒中量表(NIHSS)评分分析将其分为轻度组33例、中度组35例、重度组18例,另选取体检健康者85例作为对照组。采用酶联免疫吸附法(ELISA)检测研究对象外周血STAT6水平,实时荧光定量PCR测定外周血PPARαmRNA水平,全自动生化分析仪检测血液相关生化指标,全自动血压仪测量血压指标;Pearson分析ACI患者STAT6、PPARα水平与入院时NIHSS评分相关性。结果对照组与ACI组的甘油三酯、低密度脂蛋白胆固醇、脂蛋白、舒张压、收缩压差异具有统计学意义(P<0.05);ACI组外周血STAT6、PPARα水平在卒中1 d、3 d、7 d呈逐渐降低的趋势,且在卒中7 d时水平达到最低,两者水平在卒中14 d时有所升高,但仍低于对照组水平(P<0.05);ACI患者外周血STAT6和PPARα表达水平在卒中不同时间点均随病情程度的加重呈现逐渐降低的趋势;Pearson相关分析显示,ACI患者卒中1 d外周血STAT6和PPARα水平与NIHSS评分呈负相关,外周血STAT6与PPARα水平呈正相关(P<0.05);ACI患者卒中不同时间点,预后良好组STAT6、PPARα水平均显著高于预后不良组(P<0.05)。结论ACI发生后患者外周血STAT6、PPARα水平均降低,且随着卒中时间的推移两者水平呈现先下降后上升的趋势,STAT6、PPARα水平变化与ACI患者的病情严重程度及预后情况均有关。Objective To investigate the dynamic changes of signal transducer and activator of transcription 6(STAT6)and peroxisome proliferator-activated receptor alpha(PPARα)in peripheral blood of patients with acute cerebral infarction(ACI)and their association with disease severity and prognosis.Methods A total of 86 ACI patients who were hospitalized in our hospital from January 2021 to June 2022 were enrolled as ACI group,and according to the National Institutes of Health Stroke Scale(NIHSS)score,they were divided into mild group with 33 patients,moderate group with 35 patients,and severe group with 18 patients;a total of 85 individuals who underwent physical examination were enrolled as control group.ELISA was used to measure the level of STAT6 in peripheral blood;quantitative real-time PCR was used to measure the mRNA level of PPARαin peripheral blood;an automatic biochemical analyzer was used to measure blood-related biochemical parameters,and an automatic blood pressure instrument was used to measure blood pressure parameters;a Pearson correlation analysis was used to investigate the correlation of STAT6 and PPARαlevels with NIHSS score on admission.Results There were significant differences between the control group and the ACI group in triglycerides,low-density lipoprotein cholesterol,lipoprotein,diastolic pressure,and systolic pressure(P<0.05).The ACI group showed gradual reductions in the levels of STAT6 and PPARαin peripheral blood on days 1,3,and 7 of stroke,with the lowest levels observed on day 7 of stroke,and there were increases in the levels of STAT6 and PPARαon day 14 of stroke,which was still lower than the levels in the control group(P<0.05).For the ACI patients,the expression levels of STAT6 and PPARαin peripheral blood tended to decrease gradually with the aggravation of disease conditions at different time points of stroke.The Pearson correlation analysis showed that the levels of STAT6 and PPARαin peripheral blood of ACI patients on day 1 of stroke were negatively correlated with NIHSS scor

关 键 词：急性脑梗死 信号转导及转录激活因子6 过氧化物酶体增殖物激活受体Α 动态变化 预后 

分 类 号：R743.3[医药卫生—神经病学与精神病学]