复合酶强化水解排水管网沉积物  

Enhancement of the sediment hydrolysis in drainage network assisted by compound enzyme

作　　者：袁春波[1] 齐伟[1] 邱文韬 李一兵 张彦平 白珺 YUAN Chunbo;QI Wei;QIU Wentao;LI Yibing;ZHANG Yanping;BAI Jun(Tianjin Water Conservancy Research Institute,Tianjin 300061;School of Civil and Transportation Engineering,Hebei University of Technology,Tianjin 300401)

机构地区：[1]天津市水利科学研究院,天津300061 [2]河北工业大学,土木与交通学院,天津300401

出　　处：《环境科学学报》2024年第5期243-252,共10页Acta Scientiae Circumstantiae

基　　金：天津市水利科学研究院横向课题(No.HE2165)。

摘　　要：采用淀粉酶复配中性蛋白酶水解排水管网沉积物以达到延缓管网淤堵的问题.在复合酶(α-淀粉酶∶中性蛋白酶=2∶3)投加量8%(W/W),pH 7,25℃条件下反应2 h时,溶解性化学需氧量(SCOD)由原泥的189.5 mg·L^(-1)上升到4026.48 mg·L^(-1),多糖从18.12 mg·L^(-1)升高到1766.54mg·L^(-1),氨氮由32.97 mg·L^(-1)提升到72.94 mg·L^(-1),有机质含量由58.23%减少到52.36%.扫描电子显微镜(SEM)、胞外聚合物(EPS)及三维荧光光谱(EEMs)结果显示,经复合酶处理后沉积物的表面结构变的较为松散,内部物质溶出;EPS含量变化为紧密型胞外聚合物(TB-EPS)>溶解型胞外聚合物(S-EPS)>疏松型胞外聚合物(LB-EPS);腐殖酸类物质由内向外转移,液相中蛋白类、可溶性微生物副产物增多,且可溶性产物荧光强度是经单一中性蛋白酶处理后的2~3倍.该结果表明,复合酶有效促进了沉积物结构的破坏与水解程度的提高,从而便于沉积物被水力冲刷,延缓了管网的淤堵.Sediment accumulation in drainage networks poses a significant challenge,leading to pipe blockage issues.In this work,amylase in combination with neutral protease was adopted to hydrolyze the sediment in drainage network to alleviate pipe blockage problem.When the hydrolysis reaction lasted for 2 h with compound enzyme(α-amylase:neutral protease =2∶3) dosage of 8%(W/W),pH 7 and 25 ℃,resulting in increased Soluble Chemical Oxygen Demand(SCOD) from 189.5 mg·L^(-1) to 4026.48 mg·L^(-1),polysaccharide content from 18.12 mg·L^(-1) to 1766.54 mg·L^(-1),ammonia nitrogen from 32.97 mg·L^(-1) to 72.94 mg·L^(-1) and decreased organic matter content from 58.23% to 52.36%.Scanning Electron Microscopy(SEM) results showed that a looser surface structure and dissolution of internal substances.The change in Extracellular Polymeric Substances(EPS) content was Tightly Bound EPS(TB-EPS) > Soluble-EPS(S-EPS) > Loosely Bound EPS(LB-EPS).The Excitation-Emission-Matrix Spectra(EEMs) analysis results showed that humic acids were transferred from the interior to the exterior of sediment particles.The fluorescence intensity of soluble products was 2~3 times higher compared to single neutral protease treatment,indicating increased protein and soluble microbial byproducts.The results of this study indicated that compound enzymes could effectively promote the destruction of sediment structure and enhance hydrolysis,facilitating hydraulic flushing and mitigating pipe blockage problem in drainage networks.

关 键 词：中性蛋白酶 淀粉酶 排水管网 沉积物 水解 

分 类 号：TU992[建筑科学—市政工程]