灵芝复合剂乙酸乙酯提取物的化学组成及其抗肿瘤活性  

Chemical Composition and Antitumor Activity of Ethyl Acetate Extracts from Ganoderma lucidum Compounds

作　　者：林晓亮 李嘉辉[2] 龙洁怡 梁明 林洪宇 张慧婷 安泗谕 刘果[2] 曹庸[2] LIN Xiaoliang;LI Jiahui;LONG Jieyi;LIANG Ming;LIN Hongyu;ZHANG Huiting;AN Siyu;LIU Guo;CAO Yong(Infinitus China Co.Ltd.,Guangzhou 510403,China;College of Food Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,Guangdong Natural Active Object Engineering Technology Research Center,Guangzhou 510642,China)

机构地区：[1]无限极(中国)有限公司,广东广州510403 [2]华南农业大学食品学院,广东省功能食品活性物重点实验室,广东省天然活性物工程技术研究中心,广东广州510642

出　　处：《现代食品科技》2024年第5期15-23,共9页Modern Food Science and Technology

基　　金：广东省自然科学基金-面上项目(2020A1515010371)。

摘　　要：该文研究了灵芝复合剂乙酸乙酯提取物(Ganoderma lucidum Complex Ethyl Acetate Extract,GCE)的化学组成及抗肿瘤活性。GCE由灵芝、菟丝子、五味子、党参(质量比50.3:25.2:15.7:8.8)组成复合剂后再经乙酸乙酯萃取所得。试验采用超高压液相色谱飞行时间质谱联用仪(UPLC-Q-TOF-MS)分析其物质组成;设空白组、模型组、阳性对照组、GCE低、中、高剂量组。检测其抑瘤率,脏器指数,脾淋巴细胞增殖能力,血清细胞因子。结果表明:GCE主要由11种化合物组成:L-焦谷氨酸、DL-亮氨酸腺苷、D-赤酮酸内酯、L-正亮氨酸、邻苯三酚、2-羟基-3-甲基吡喃-4-酮、绿原酸、3-(3,4,5-三甲氧基苯基)丙酸、槲皮素、五味子素;GCE低、中、高剂量组(50、100、200 mg/kg)H22荷瘤小鼠的抑瘤率分别为35.14%、38.25%、50.87%;与模型组相比,GCE低、中、高剂量均能显著促进小鼠脾淋巴细胞的增殖(P<0.01);GCE高剂量能显著促进小鼠的IL-6、TNF-α和IL-12分泌(P<0.05)。结论,GCE对H22荷瘤小鼠肿瘤有一定抑制作用,该研究结果可为抗肿瘤功能的产品研发提供新思路。The chemical composition and antitumor activity of ethyl acetate extracts from Ganoderma lucidum compounds(GCE)were investigated.GCE was extracted using ethyl acetate from compounds of Ganoderma lucidum,Cuscuta chinensis Lam,Schisandra chinensis,and Codonopsis pilosula(at a mass ratio of 50.3:25.2:15.7:8.8).Ultra-high pressure liquid chromatography coupled with time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze its composition.Blank,model,positive control,and GCE-treated groups(low,medium,and high dosages)were established.The tumor inhibition rate,organ index,splenic lymphocyte proliferation capacity,and serum cytokine levels were measured.The results showed that GCE is mainly composed of 11 compounds:L-pyroglutamic acid,DL-leucine adenosine,D-erythronolactone,L-norleucine,pyrogallol,2-hydroxy-3-methylpyran-4-one,chlorogenic acid,3-(3,4,5-trimethoxyphenyl)propionic acid,quercetin,and schisandrin.The tumor inhibition rates of H22 tumor-bearing mice in low-,medium-,and high-dose GCE-treated groups(at 50,100 and 200 mg/kg)H22 were 35.14%,38.25%,and 50.87%,respectively.Comparison of the model and treated groups revealed that low-,medium-,and high-dose GCE significantly promoted the proliferation of splenic lymphocytes(P<0.01).A high dose of GCE could significantly promote the secretion of IL-6,TNF-α,and IL-12 in mice(P<0.05).In short,GCE has certain inhibitory effects on the tumor of H22 tumor-bearing mice.The results of this study can provide insights into the development of antitumor products.

关 键 词：灵芝复合剂乙酸乙酯提取物 化学组成 小鼠模型 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]