产蛋白酶波茨坦短芽孢杆菌的鉴定及产酶条件优化  

Identification of a Protease Producing Brevibacillus borstelensis Strain and Optimization of Enzyme Production Conditions

作　　者：梁安健 石沁兰 王金丽 朱成林 邹立扣 朱鹏程 李东亮 唐俊妮[1] LIANG Anjian;SHI Qinlan;WANG Jinli;ZHU Chenglin;ZOU Likou;ZHU Pengcheng;LI Dongliang;TANG Junni(College of Food Sciences and Technology,Southwest Minzu University,Chengdu 610041,China;College of Resources,Sichuan Agricultural University,Chengdu 611830,China;Sichuan China Tobacco Industry Co.Ltd.,Chengdu 610021,China)

机构地区：[1]西南民族大学食品科学与技术学院,四川成都610041 [2]四川农业大学资源学院,四川成都611830 [3]四川中烟工业有限责任公司,四川成都610021

出　　处：《现代食品科技》2024年第5期73-83,共11页Modern Food Science and Technology

基　　金：四川省重点实验室开放课题项目(川烟工技[2022]220号)。

摘　　要：该研究旨在提高一株环境分离菌株的产酶效率,为后续菌株及其蛋白酶的应用提供前期实验基础。通过水解圈法初步检测菌株S8的产蛋白酶能力,并通过16S rRNA序列比对确认其为波茨坦短芽孢杆菌。通过单因素试验确定了最佳培养条件和培养基添加成分。并采用Plackett-Burman设计和最陡爬坡试验对培养条件和培养基进行响应面优化。优化结果显示,在发酵时间为38.70 h、菌液接种量为1.84%(V/V)、发酵温度为35℃、酵母粉添加量为23.70 g/L、胰蛋白胨添加量为11.70 g/L、MgSO4添加量为20.20 g/L的条件下,菌株的产酶活力可达到114.79 U/mL,相比优化前提升了209.70%。研究结果为该菌株的后续发酵应用提供了科学数据。In order to improve the enzyme production efficiency of an environmentally isolated strain,and provide a preliminary experimental basis for the subsequent application of the strain and its protease,the protease production ability of strain S8 was preliminarily detected using the hydrolysis circle method,and its identity as Brevibacillu sborstelensis was confirmed by comparing the 16S rRNA sequences.The optimal culture conditions and medium components were determined through single-factor experiments.Finally,the culture conditions and medium were further optimized using response surface methodology with the help of Plackett-Burman design and the steepest ascent method.The optimization results showed that the optimal conditions are as follows:a fermentation time of 38.70 h,an inoculum size of 1.84%(V/V),a fermentation temperature at 35℃,a yeast powder addition of 23.70 g/L,a pancreatic digest of casein addition of 11.70 g/L,and a MgSO4 addition of 20.20 g/L.The resulting enzyme production activity of the strain reached 114.79 U/mL,which was 209.70%higher than that under non-optimized conditions.The results of this study provide scientific data for the subsequent fermentation application of this strain.

关 键 词：波茨坦短芽孢杆菌 产蛋白酶 产酶条件优化 响应面法 

分 类 号：TS201.3[轻工技术与工程—食品科学]